Zheng Jin-ping, Sun Jian-ya, Guo Liang, Liang Hua-shan, Tian Feng-jie, Wu Tang-chun
Department of Toxicology, Shanxi Medical University, Taiyuan 030001, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Jul;25(7):394-7.
To investigate the relationship between heat shock protein 72 (Hsp72) and DNA genetic damage in peripheral blood lymphocytes of coke oven workers and the role of Hsp72 in protection of cells from genetic damage induced by coke oven emissions.
Two hundred and sixty-seven coke oven workers and thirty controls without occupational PAHs exposure were investigated. Benzo[a]pyrene concentrations in the ambient air individually collected were assayed by high performance liquid chromatography (HPLC). Western Blot was used to measure Hsp72 levels and Comet assay was used to evaluate DNA damage degree. Personal information was collected by questionnaire.
The Hsp72 level (G+/-S(G)) and olive comet tail moment (G+/-S(G) of peripheral blood lymphocytes in high-exposure workers (1.24 +/- 0.42 and 4.49 +/- 1.24) were significantly higher than those in low-exposure workers (1.01 +/- 0.35 and 2.99 +/- 1.10, P < 0.05) and control (0.85 +/- 0.34 and 2.40 +/- 1.00, P < 0.05) respectively. The Hsp72 median level of all subjects was used as the limit to divide subjects into high Hsp72 level group and low Hsp72 level group. The rate with high Hsp72 level was 36.7%, 43.1% and 58.3% in control, low exposure and high exposure workers respectively and had a rising tendency following exposure level (P = 0.003). In high Hsp72 level group Hsp72 level in high exposure workers was significantly higher than that in control (P < 0.05), and there was a rising tendency along with the increase of exposed levels. But the olive comet tail moment had no significant difference among three exposed groups (P > 0.05). In low Hsp72 level group there no difference among three exposed groups about Hsp72 levels. The olive comet tail moment in high exposure workers was significantly higher than that in low exposure workers and control (P < 0.01) and high exposure workers in Hsp72 positive group and there was a rising tendency along with the increase of exposed levels. Hsp72 levels had strong negative correlation with the olive comet tail moment (r = -0.503, P < 0.01) in high exposure workers.
The coke oven emissions can induce hsp72 expression. Hsp72 play a role of protecting cells from DNA damage induced by coke oven emissions.
探讨焦炉工人外周血淋巴细胞中热休克蛋白72（Hsp72）与DNA遗传损伤的关系以及Hsp72在保护细胞免受焦炉排放物诱导的遗传损伤中的作用。
对267名焦炉工人和30名无职业多环芳烃暴露的对照者进行调查。采用高效液相色谱法（HPLC）测定个体采集的环境空气中苯并[a]芘浓度。采用蛋白质免疫印迹法检测Hsp72水平，采用彗星试验评估DNA损伤程度。通过。通过问卷调查收集个人信息。
高暴露组工人外周血淋巴细胞中Hsp72水平（G+/-S(G)）和橄榄彗星尾矩（G+/-S(G)）分别为（1.24±0.42和4.49±1.24），显著高于低暴露组工人（1.01±0.35和2.99±1.10，P<0.05）和对照组（0.85±0.34和2.40±1.00，P<0.05）。以所有受试者Hsp72水平中位数为界将受试者分为Hsp72高水平组和低水平组。对照组、低暴露组和高暴露组工人中Hsp72高水平率分别为36.7%、43.1%和58.3%，且随暴露水平升高呈上升趋势（P=0.003）。在Hsp72高水平组中，高暴露组工人的Hsp72水平显著高于对照组（P<0.05），且随暴露水平升高呈上升趋势。但三个暴露组间橄榄彗星尾矩差异无统计学意义（P>0.05）。在Hsp72低水平组中，三个暴露组的Hsp72水平无差异。高暴露组工人的橄榄彗星尾矩显著高于低暴露组工人和对照组（P<0.01），且Hsp72阳性组中高暴露组工人的橄榄彗星尾矩随暴露水平升高呈上升趋势。高暴露组工人中Hsp72水平与橄榄彗星尾矩呈强负相关（r=-0.503，P<0.01）。
焦炉排放物可诱导Hsp72表达。Hsp72在保护细胞免受焦炉排放物诱导的DNA损伤中发挥作用。
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