Leng Shu-guang, Zheng Yu-xin, Zhang Wen-zhong, Dai Yu-fei, Niu Yong, Wang Ya-wen, Gu Ye-ping, Pan Zu-fei, Xiao Jun, Wang Zhong-xu, Li Tao, He Feng-sheng
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2004 Feb;22(1):29-32.
To investigate DNA and chromosome damage in peripheral blood lymphocyte of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs).
One hundred and thirty-seven coke oven workers and 50 controls without occupational PAHs exposure were investigated. Comet assay and cytokinesis-block micronucleus (CBMN) detection were used to evaluate DNA and chromosomal damage levels in peripheral blood lymphocyte. Urinary 1-hydroxypyrene level was used to assess the personal internal PAHs exposure dose. Personal information including occupational history, age, sex, smoking and drinking status was collected by questionnaire.
Urinary 1-hydroxypyrene level in coke oven workers [(5.76 +/- 1.04) micro mol/mol Cr] was significantly higher than that in controls [(0.70 +/- 0.32) micro mol/mol Cr]. The rate of CBMN and comet tail moment of lymphocyte in coke oven workers [8.0 per thousand (0.0 per thousand - 30.0 per thousand ) and 2.09 (0.31 - 75.41), respectively] were higher than those in controls [3.5 per thousand (0.0 per thousand - 13.0 per thousand ) and 1.05 (0.11 - 6.63), P < 0.05]. In controls, the comet moment in smokers was significantly higher than that in non-smokers [1.44 (0.23 - 6.63) vs 0.81 (0.11 - 3.47), P < 0.05]. According to the length of work, 137 coke oven workers were classified into 3 groups i.e. 0.5 yrs , 16.0 yrs and 22.0 yrs group, and the comet moments were 1.34 (0.31 - 37.84), 2.32 (0.49 - 52.97) and 3.20 (0.45 - 75.41) respectively after adjusting the age, sex, smoking, drinking and level of urinary 1-hydroxy-pyrene. There was a rising tendency along with the increase in length of work.
Under present PAHs exposure levels, both comet assay and Cytokinesis-block micronucleus test could detect PAHs-induced genotoxicity in coke oven workers, and comet assay is more suitable to assess the cumulative damage effect on DNA.
研究接触多环芳烃（PAHs）的焦炉工人外周血淋巴细胞中的DNA和染色体损伤情况。
对137名焦炉工人和50名无职业性PAHs接触的对照者进行调查。采用彗星试验和胞质分裂阻滞微核（CBMN）检测法评估外周血淋巴细胞中的DNA和染色体损伤水平。尿中1-羟基芘水平用于评估个人体内PAHs接触剂量。通过问卷调查收集包括职业史、年龄、性别、吸烟和饮酒状况在内的个人信息。
焦炉工人尿中1-羟基芘水平[（5.76±1.04）μmol/mol肌酐]显著高于对照组[（0.70±0.32）μmol/mol肌酐]。焦炉工人淋巴细胞的CBMN率和彗星尾矩[分别为8.0‰（0.0‰-30.0‰）和2.09（0.31-75.41）]高于对照组[3.5‰（0.0‰-13.0‰）和1.05（0.11-6.63），P<0.05]。在对照组中，吸烟者的彗星矩显著高于非吸烟者[1.44（0.23-6.63）对0.81（0.11-3.47），P<0.05]。根据工作年限，将137名焦炉工人分为3组，即0.5年组、16.0年组和22.0年组，在调整年龄、性别、吸烟、饮酒和尿中1-羟基芘水平后，彗星矩分别为1.34（0.31-37.84）、2.32（0.49-52.97）和3.20（0.45-75.41）。随着工作年限的增加呈上升趋势。
在目前的PAHs接触水平下，彗星试验和胞质分裂阻滞微核试验均可检测到焦炉工人中PAHs诱导的遗传毒性，且彗星试验更适合评估对DNA的累积损伤效应。
