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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
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Current developments in peanut allergy.花生过敏的当前进展
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Likelihood-enhanced fast translation functions.似然增强快速翻译功能。
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Peanut allergenicity.花生致敏性。
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Refinement of macromolecular structures by the maximum-likelihood method.用最大似然法优化大分子结构。
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Storage protein accumulation in the absence of the vacuolar processing enzyme family of cysteine proteases.在缺乏半胱氨酸蛋白酶的液泡加工酶家族的情况下储存蛋白的积累。
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Peanut allergen Ara h 3: isolation from peanuts and biochemical characterization.花生过敏原Ara h 3：从花生中分离及生化特性分析
Allergy. 2003 Nov;58(11):1144-51. doi: 10.1034/j.1398-9995.2003.00259.x.
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Crystal structure of soybean 11S globulin: glycinin A3B4 homohexamer.大豆11S球蛋白：大豆球蛋白A3B4同型六聚体的晶体结构
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花生主要过敏原Ara h 3的纯化、结晶及初步晶体学表征

Purification, crystallization and initial crystallographic characterization of peanut major allergen Ara h 3.

作者信息

Jin Tengchuan, Howard Andrew, Zhang Yu-Zhu

机构信息

Department of Biology, Illinois Institute of Technology, Chicago, IL 60616, USA.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt 10):848-51. doi: 10.1107/S1744309107041176. Epub 2007 Sep 19.

DOI:10.1107/S1744309107041176
PMID:17909286
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2339721/
Abstract

The peanut is a significant food source, but is responsible for many cases of anaphylaxis. The peanut 11S legumin-like seed storage protein Ara h 3 is one of the best characterized allergens. In this study, Ara h 3 was extracted from peanut kernels and purified by sequential anion-exchange, hydrophobic interaction and gel-filtration chromatography to very high purity to facilitate crystallization and structural studies. Well diffracting single crystals were obtained by the vapor-diffusion method. A molecular-replacement structural solution has been obtained and refinement of the structure is currently under way.

摘要

花生是一种重要的食物来源，但会引发许多过敏反应病例。花生11S类豆球蛋白种子贮藏蛋白Ara h 3是特征最明确的过敏原之一。在本研究中，从花生仁中提取Ara h 3，并通过连续的阴离子交换、疏水相互作用和凝胶过滤色谱法将其纯化至非常高的纯度，以利于结晶和结构研究。通过气相扩散法获得了衍射良好的单晶。已获得分子置换结构解析，目前正在对结构进行精修。