黑腹果蝇β-丙氨酸合酶的结晶及初步X射线数据分析
Crystallization and preliminary X-ray data analysis of beta-alanine synthase from Drosophila melanogaster.
作者信息
Lundgren Stina, Andersen Birgit, Piskur Jure, Dobritzsch Doreen
机构信息
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt 10):874-7. doi: 10.1107/S1744309107042984. Epub 2007 Sep 19.
Abstract
Beta-alanine synthase catalyzes the last step in the reductive degradation pathway for uracil and thymine, which represents the main clearance route for the widely used anticancer drug 5-fluorouracil. Crystals of the recombinant enzyme from Drosophila melanogaster, which is closely related to the human enzyme, were obtained by the hanging-drop vapour-diffusion method. They diffracted to 3.3 A at a synchrotron-radiation source, belong to space group C2 (unit-cell parameters a = 278.9, b = 95.0, c = 199.3 A, beta = 125.8 degrees) and contain 8-10 molecules per asymmetric unit.
摘要
β-丙氨酸合酶催化尿嘧啶和胸腺嘧啶还原降解途径的最后一步，而该途径是广泛使用的抗癌药物5-氟尿嘧啶的主要清除途径。通过悬滴气相扩散法获得了与人类酶密切相关的黑腹果蝇重组酶的晶体。它们在同步辐射源处衍射至3.3 Å，属于空间群C2（晶胞参数a = 278.9，b = 95.0，c = 199.3 Å，β = 125.8°），每个不对称单元包含8-10个分子。
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