Moon Young Jin, Zhang Shuzhong, Morris Marilyn E
Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Amherst, NY 14260-1200, USA.
Acta Haematol. 2007;118(3):169-75. doi: 10.1159/000109093. Epub 2007 Oct 2.
The expression levels of mRNAs for MDR1 (P-glycoprotein), multidrug resistance-associated proteins (MRP1), and BCRP (breast cancer resistance protein; mitoxantrone resistance protein) were quantitatively determined in lymphocytes and monocytes. Monocytes and lymphocytes were obtained from 3 healthy male and 2 healthy female volunteers. BCRP, MDR1, and MRP1 mRNA levels were determined by real-time quantitative reverse transcriptase polymerase chain reaction. In lymphocytes, relative concentrations of mRNAs for target proteins (BCRP, MDR1, and MRP1) were 1.00 +/- 0.478, 9.67 +/- 5.53, and 0.116 +/- 0.0613 respectively, and in monocytes 1.00 +/- 0.854, 0.821 +/- 0.263, and 0.090 +/- 0.052, respectively. The MDR1 mRNA level was cell type dependent, whereas there was no difference in BCRP and MRP1 expression levels between lymphocytes and monocytes. Comparison of mRNA levels for the three major multidrug-resistant efflux pumps reveals that MDR1 is the predominant form in lymphocytes and BCRP is the predominant form in monocytes.
在淋巴细胞和单核细胞中定量测定了多药耐药蛋白1（P-糖蛋白）、多药耐药相关蛋白（MRP1）和乳腺癌耐药蛋白（BCRP；米托蒽醌耐药蛋白）的mRNA表达水平。单核细胞和淋巴细胞取自3名健康男性和2名健康女性志愿者。通过实时定量逆转录聚合酶链反应测定BCRP、MDR1和MRP1的mRNA水平。在淋巴细胞中，靶蛋白（BCRP、MDR1和MRP1）的mRNA相对浓度分别为1.00±0.478、9.67±5.53和0.116±0.0613，在单核细胞中分别为1.00±0.854、0.821±0.263和0.090±0.052。MDR1的mRNA水平取决于细胞类型，而淋巴细胞和单核细胞之间BCRP和MRP1的表达水平没有差异。对三种主要多药耐药外排泵的mRNA水平进行比较发现，MDR1是淋巴细胞中的主要形式，而BCRP是单核细胞中的主要形式。
