The iron deficiency is a major problem of public health in the African countries, particularly in pregnant women population. The aim of our study was to appreciate the iron status during the pregnancy period (first, second and three trimesters); to realize an evaluation and to characterize the biological indicators of pregnant women. Our study was carried out in four medical and urban units of Abidjan in Côte-d'Ivoire with 531 pregnant women. The biological parameters significantly reduced in the third trimester of the pregnancy. But, an enhancement of the transferrin and the total iron binding capacity was observed. Moreover, 66 % of pregnant women presented iron deficiency anaemia (p < 0.01). In conclusion, the study shows that no pregnant woman presented iron normal status in the third trimester of the pregnancy.