大肠杆菌中deoP1启动子和操纵子突变体：分离与特性分析

deoP1 promoter and operator mutants in Escherichia coli: isolation and characterization.

作者信息

Dandanell G, Hammer K

机构信息

Institute of Biological Chemistry B, University of Copenhagen, Denmark.

出版信息

Mol Microbiol. 1991 Oct;5(10):2371-6. doi: 10.1111/j.1365-2958.1991.tb02083.x.

DOI:10.1111/j.1365-2958.1991.tb02083.x

PMID:1791752

Abstract

Plasmid DNA containing deoP1, one of the two major promoters of the deo operon, has been mutagenized using hydroxylamine, and promoter down-mutations and operator mutations were selected. The isolated mutants are all located within a 16 bp palindromic sequence containing the -10 region of deoP1. The results show that RNA polymerase and DeoR repressor compete for the same DNA target. The deoP1 promotor activity is dependent on a TG motif one base pair upstream of the -10 consensus sequence. The sequence of the deo operator site was further verified by use of a synthetic linker.

摘要

含有deo操纵子两个主要启动子之一deoP1的质粒DNA已用羟胺诱变，并筛选出启动子下调突变和操纵基因突变。分离出的突变体均位于一个16bp的回文序列内，该序列包含deoP1的-10区。结果表明，RNA聚合酶和DeoR阻遏物竞争相同的DNA靶标。deoP1启动子活性依赖于-10共有序列上游一个碱基对处的TG基序。通过使用合成接头进一步验证了deo操纵位点的序列。