[环形泰勒虫Tams1基因的聚合酶链反应-限制性片段长度多态性分析]
[PCR-RFLP analysis of the Tams1 gene of Theileria annulata].
作者信息
Altay Kürşat, Aktaş Münir, Dumanli Nazir
机构信息
Firat Universitesi, Veteriner Fakültesi Parazitoloji Anabilim Dali, Elaziğ, Turkey.
出版信息
Turkiye Parazitol Derg. 2007;31(3):173-5.
Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELISA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
Tams1是环形泰勒虫的裂殖子表面抗原。Tams1的基因变异使得疫苗研究以及像酶联免疫吸附测定（ELISA）这样的诊断测试变得困难。在本研究中，对从埃拉泽省和宾格尔省自然感染牛身上获取的89株环形泰勒虫分离株的Tams1基因进行了聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测。检测到六种不同的限制性图谱（a、b、c、d、e、f）。89个样本的限制性图谱数量如下：78个（a）、2个（b）、2个（c）、5个（d）、1个（e）和1个（f）。
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