Hutson K A, Durham D, Tucci D L
Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, Duke University Medical Center, Box 3805, Durham, NC 27710, USA.
Hear Res. 2007 Nov;233(1-2):124-34. doi: 10.1016/j.heares.2007.08.003. Epub 2007 Aug 30.
Conductive hearing impairment results in marked changes in neuronal activity in the central auditory system, particularly in young animals [Tucci, D.L., Cant, N.B., Durham, D., 1999. Conductive hearing loss results in a decrease in central auditory system activity in the young gerbil. Laryngoscope 109, 1359-1371]. To better understand the effects of conductive hearing loss (CHL) on cellular metabolism, incorporation of (3)H-leucine was used as a measure of protein synthesis in immature postnatal day 21 gerbils subjected to either unilateral CHL by malleus removal or profound sensorineural hearing loss by cochlear ablation. (3)H-leucine uptake was measured after survival times of 6 or 48h. Protein synthesis values were standardized to measurements from the abducens nucleus and compared with measurements from sham animals at similar age/survival times. Protein synthesis in the medial superior olive (MSO) was found to be significantly down-regulated (bilaterally) after CHL in animals surviving 48h. However, 6h after CHL manipulation, protein synthesis is up-regulated in MSO (bilaterally) and in the ipsilateral medial nucleus of the trapezoid body.
传导性听力损失会导致中枢听觉系统神经元活动发生显著变化，尤其是在幼龄动物中[图奇，D.L.，坎特，N.B.，达勒姆，D.，1999年。传导性听力损失导致幼年沙鼠中枢听觉系统活动减少。喉镜109，1359 - 1371]。为了更好地理解传导性听力损失（CHL）对细胞代谢的影响，采用（3）H - 亮氨酸掺入法来测量出生后第21天的未成熟沙鼠的蛋白质合成，这些沙鼠要么通过去除锤骨造成单侧CHL，要么通过耳蜗切除造成严重的感音神经性听力损失。在6小时或48小时的存活时间后测量（3）H - 亮氨酸摄取量。蛋白质合成值根据展神经核的测量值进行标准化，并与相同年龄/存活时间的假手术动物的测量值进行比较。发现在存活48小时的动物中，CHL后内侧上橄榄核（MSO）的蛋白质合成显著下调（双侧）。然而，在CHL操作6小时后，MSO（双侧）和同侧梯形体内侧核的蛋白质合成上调。
