Expression of two testis-specific genes, TSGA10 and SYCP3, in different cancers regarding to their pathological features.

BACKGROUND

Cancer-testis genes are a group of genes expressed in testicular germinal cells and a range of human cancers. Testis-specific gene A10 (TSGA10) is expressed in testis and actively dividing and fetal differentiating tissues. Mouse homologue (Tsga10) mRNA is translated to a 65kDa protein and appears to be processed to a major fibrous sheath protein of sperm tail. SYCP3 gene is supposed to be a testis-specific gene and constitutes the core of the lateral elements of synaptonemal complex. It has role in regulating DNA binding to the chromatid axis, sister chromatid cohesion, synapsis, and recombination.

METHODS

In this study expression of TSGA10 and SYCP3 were investigated in different cancers (156 tumor samples) using RT-PCR. Diagnosis of cancer was based on histopathological reports. The association with histopathological characteristics of tumors was analyzed using statistical programs.

RESULTS

TSGA10 expression was observed in 83% of brain tumors, 66% of breast cancers, 58% of gastrointestinal tumors, 66% of skin tumors and 53% of soft tissue tumors. But, SYCP3 transcripts were found in four tumor samples (moderately differentiated gemistocytic astrocytoma, pituitary adenoma, glioma and an ovarian tumor).

CONCLUSION

These results may get further insight into TSGA10, but not SYCP3, potential role as a cancer marker and a cancer testis gene implicated in tumorogenesis of cancers.