粘球菌在子实体形成过程中的空间组织。
Spatial organization of Myxococcus xanthus during fruiting body formation.
作者信息
Curtis Patrick D, Taylor Rion G, Welch Roy D, Shimkets Lawrence J
机构信息
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
出版信息
J Bacteriol. 2007 Dec;189(24):9126-30. doi: 10.1128/JB.01008-07. Epub 2007 Oct 5.
Abstract
Microcinematography was used to examine fruiting body development of Myxococcus xanthus. Wild-type cells progress through three distinct phases: a quiescent phase with some motility but little aggregation (0 to 8 h), a period of vigorous motility leading to raised fruiting bodies (8 to 16 h), and a period of maturation during which sporulation is initiated (16 to 48 h). Fruiting bodies are extended vertically in a series of tiers, each involving the addition of a cell monolayer on top of the uppermost layer. A pilA (MXAN_5783) mutant produced less extracellular matrix material and thus allowed closer examination of tiered aggregate formation. A csgA (MXAN_1294) mutant exhibited no quiescent phase, aberrant aggregation in phase 2, and disintegration of the fruiting bodies in the third phase.
摘要
运用显微电影摄影术来研究黄色黏球菌子实体的发育。野生型细胞历经三个不同阶段：静止期，有一定运动性但很少聚集（0至8小时）；活跃运动期，导致子实体隆起（8至16小时）；成熟期，在此期间开始孢子形成（16至48小时）。子实体在一系列层次中垂直延伸，每个层次都涉及在最上层之上添加一层细胞。pilA（MXAN_5783）突变体产生的细胞外基质物质较少，因此能够更仔细地观察分层聚集体的形成。csgA（MXAN_1294）突变体没有静止期，在第二阶段聚集异常，在第三阶段子实体解体。
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