Zhu Yong-sheng, Huo Zheng-hao, Yu Bing, Zhang Hong-bo, Wang Yu-jiong, Zhao Wei, Jiao Hai-yan, Dang Jie, Li Sheng-bin
Key Laboratory of Reproduction and Genetics in Ningxia, Department of Medical Genetics and Cell Biology, Ningxia Medical College, Yingchuan, Ningxia, 750004 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Oct;24(5):594-7.
To obtain the genetic polymorphism of Y chromosomal short tandem repeat (Y-STR) loci in Ningxia Hui population.
Blood samples were collected from 150 unrelated healthy male individuals of Ningxia Hui ethnic group. Twelve Y-STR loci were amplified in one tube by using the PowerPlex System STR Amplification Kit, and the genotypes were determined using Genescan and Genotype software of ABI377 DNA sequencer and the frequency of alleles and haplotypes of Ningxia Hui ethnic was obtained.
Seventy-five alleles were observed at 12 Y-STR loci. The frequency ranged from 0.0067-0.7067 and the gene diversity ranged from 0.4446-0.8877. Totally 148 different haplotypes were found, which were unique in 150 males. Two haplotypes were shared by 2 males respectively. The haplotype diversity was 0.9864.
The 12 Y-STR loci are highly polymorphic in Ningxia Hui population and are suitable for genetics and forensic research.
获取宁夏回族人群Y染色体短串联重复序列（Y-STR）基因座的遗传多态性。
采集150名宁夏回族无血缘关系的健康男性个体的血液样本。使用PowerPlex系统STR扩增试剂盒在一管中对12个Y-STR基因座进行扩增，采用ABI377 DNA测序仪的Genescan和Genotype软件确定基因型，并获得宁夏回族人群的等位基因和单倍型频率。
在12个Y-STR基因座上共观察到75个等位基因。频率范围为0.0067 - 0.7067，基因多样性范围为0.4446 - 0.8877。共发现148种不同的单倍型，在150名男性中均为独特单倍型。有2种单倍型分别被2名男性共享。单倍型多样性为0.9864。
12个Y-STR基因座在宁夏回族人群中具有高度多态性，适用于遗传学和法医学研究。
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