[细胞周期蛋白D1和p27kip1在肾细胞癌中的表达及其意义]

[Expression of cyclin D1 and p27kip1 in renal cell carcinoma and its significance].

作者信息

Ge Jian-rong, Ren Guo-ping, Yao Hang-ping

机构信息

Department of Pathology, Shaoxing College of Arts and Sciences, Shaoxing 312000, China.

出版信息

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2007 Sep;36(5):483-7. doi: 10.3785/j.issn.1008-9292.2007.05.013.

DOI:10.3785/j.issn.1008-9292.2007.05.013

PMID:17924468

Abstract

OBJECTIVE

To investigate the role of cyclin D1 and p27(kip1) in the occurrence and development of conventional renal cell carcinoma(CRCC).

METHODS

RT-PCR and Western-blot were used to detect mRNA and protein contents of cyclin D1 and p27(kip1) in 25 CRCCs and 10 normal renal tissue distant to tumor. Immunohistochemistry was used to investigate the expression of cyclin D1 and p27(kip1) in pathological tissue sections of 76 CRCCs. The relationship between those index and clinicopathological parameters was analyzed statistically.

RESULT

In CRCC, the expression of cyclin D1 was higher than that of the control group. The higher cyclin D1 content was related to big tumor size (P<0.05); The expression of p27(kip1) was lower than that of the control group, and the lower p27(kip1) was related to higher nuclear grade and TNM stage (P<0.01). The 5-year cumulative survival rate of the p27(kip1) high expression group is longer than that of the low group (P<0.01).

CONCLUSION

The excessive expression of cyclin D1 and lower expression of p27(kip1) play an important role in the carcinogenesis of CRCC. The lower expression of p27(kip1) may affect the progression of the tumors. The detection of p27(kip1) may be as a reference marker in the prognosis of CRCC.

摘要

目的

探讨细胞周期蛋白D1（cyclin D1）和p27（kip1）在传统型肾细胞癌（CRCC）发生发展中的作用。

方法

采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western-blot）检测25例CRCC组织及10例癌旁正常肾组织中cyclin D1和p27（kip1）的mRNA及蛋白含量。采用免疫组织化学法检测76例CRCC病理组织切片中cyclin D1和p27（kip1）的表达情况，并对上述指标与临床病理参数的关系进行统计学分析。