Wu Peng, Zheng Shao-bin
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Aug;30(8):1883-6, 1889.
To investigate the expression of cyclin-dependent kinase inhibitor p27kip1 in renal cell carcinoma and its clinical significance.
The expressions of p27kip1 mRNA and protein were evaluated using reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry with tissue chip technique in renal cell carcinoma (RCC) tissues, adjacent tissues and 786-0 cell line. The relationship between the expression of p27kip1 and the tumor size, the clinical stage, pathological type and stage were evaluated, and the results by the 3 methods were analyzed to evaluate the relationship between the mRNA and protein of p27kip1.
The expression of p27kip1 protein (detected by immunohistochemistry and Western blotting) in RCC tissue was significantly lower than that in the adjacent normal tissues (P<0.05). The expression of p27kip1 mRNA (by RT-PCR) showed no significant difference between the two groups. p27kip1 protein expression was found to be inversely correlated to the tumor grade and clinical stage (P<0.05). Western blotting and immunohistochemistry showed a high coincidence rate in the detection of P27kip1 protein expression in RCC samples (P<0.001, K=0.828).
p27kip1 mRNA expression shows no difference between RCC and normal tissues, while the protein expression is significantly lower in the cancer tissues, suggesting a regulatory mechanism of p27kip1 expression at the transcriptional level. Low expression of p27kip1 protein may promote the development and progression of RCC. p27kip1 protein may serve as a diagnostic and prognostic marker of RCC.
探讨细胞周期蛋白依赖性激酶抑制剂p27kip1在肾细胞癌中的表达及其临床意义。
采用逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法以及组织芯片技术免疫组化法，检测肾细胞癌（RCC）组织、癌旁组织及786-0细胞系中p27kip1 mRNA和蛋白的表达。评估p27kip1表达与肿瘤大小、临床分期、病理类型及分级的关系，并分析3种方法的检测结果，以评估p27kip1 mRNA与蛋白之间的关系。
RCC组织中p27kip1蛋白表达（免疫组化法及蛋白质免疫印迹法检测）显著低于癌旁正常组织（P<0.05）。两组间p27kip1 mRNA表达（RT-PCR法检测）无显著差异。发现p27kip1蛋白表达与肿瘤分级及临床分期呈负相关（P<0.05）。蛋白质免疫印迹法与免疫组化法检测RCC样本中P27kip1蛋白表达的符合率较高（P<0.001，K=0.828）。
RCC与正常组织间p27kip1 mRNA表达无差异，而癌组织中蛋白表达显著降低，提示p27kip1表达在转录水平存在调控机制。p27kip1蛋白低表达可能促进RCC的发生发展。p27kip1蛋白可作为RCC的诊断及预后标志物。
