[53BP1和p53基因多态性与食管鳞状细胞癌和贲门腺癌易感性的相关性]

[Correlation of 53BP1 and p53 polymorphisms to susceptibility to esophageal squamous cell carcinoma and gastric cardiac adenocarcinoma].

作者信息

Cao Yan-Yan, Ge Hui, Chen Long-Qi, Chen Zhi-Feng, Wen Deng-Gui, Li Yan, Zhang Jian-Hui

机构信息

Laboratory of Molecular Biology, Hebei Provincial Cancer Institute/The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050011, P. R. China.

出版信息

Ai Zheng. 2007 Oct;26(10):1052-7.

PMID:17927872

Abstract

BACKGROUND & OBJECTIVE: 53BP1 is one of p53-binding proteins, which can enhance the transcriptional activation of p53 and plays a key role in tumor suppression. A single nucleotide polymorphism (SNP) T885G has been found in the promoter of 53BP1. This study was to investigate the correlation of 53BP1 and p53 SNPs to susceptibility to esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA) in a high incidence area of Hebei Province in China.

METHODS

Genotypes of 53BP1 T885G and p53 Arg72Pro in 349 ESCC patients, 275 GCA patients, and 635 healthy subjects were detected by primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR).

RESULTS

The overall distribution of 53BP1 T885G was not significantly different between ESCC patients, GCA patients and healthy subjects (P>0.05). When stratified by smoking status and family history of upper gastrointestinal cancer (UGIC), the distribution of 53BP1 T885G was not significantly different between ESCC patients, GCA patients and healthy subjects. Compared with p53 Arg72Pro Arg/Arg genotype, Pro/Pro genotype decreased the susceptibility to GCA [the age, sex, smoking status, and family history adjusted odds ratio (OR)=0.79, 95% confidence interval (CI)=0.64-0.98]. Stratification analysis showed that Pro/Pro genotype decreased the susceptibility to GCA among non-smokers (adjusted OR=0.72, 95% CI=0.54-0.97), but p53 Arg72Pro had no influence on the susceptibility to ESCC. Stratified by p53 Arg72Pro genotype, 53BP1 T885G G/G genotype reduced the susceptibility to GCA among the individuals with Pro allele (Arg/Pro and Pro/Pro genotypes) (adjusted OR=0.74, 95% CI=0.57-0.95).

CONCLUSION

53BP1 T885G may not be correlated to the susceptibility to ESCC and GCA in the high incidence area of Hebei Province in China; p53 Arg72Pro Pro/Pro genotype could decrease the susceptibility to GCA; 53BP1 T885G G/G genotype could reduce the susceptibility to GCA among the individuals with p53 Pro allele.

摘要

背景与目的

53BP1是一种与p53结合的蛋白，可增强p53的转录激活作用，在肿瘤抑制中起关键作用。已在53BP1启动子中发现单核苷酸多态性（SNP）T885G。本研究旨在探讨中国河北省一个食管癌高发地区53BP1和p53基因单核苷酸多态性与食管鳞状细胞癌（ESCC）和贲门腺癌（GCA）易感性的相关性。

方法

采用引物引入限制性分析-聚合酶链反应（PIRA-PCR）检测349例ESCC患者、275例GCA患者和635例健康对照者的53BP1 T885G和p53 Arg72Pro基因型。

结果

ESCC患者、GCA患者和健康对照者中53BP1 T885G的总体分布无显著差异（P>0.05）。按吸烟状况和上消化道癌（UGIC）家族史分层后，ESCC患者、GCA患者和健康对照者中53BP1 T885G的分布仍无显著差异。与p53 Arg72Pro Arg/Arg基因型相比，Pro/Pro基因型降低了GCA的易感性[年龄、性别、吸烟状况和家族史校正比值比（OR）=0.79，95%置信区间（CI）=0.64-0.98]。分层分析显示，Pro/Pro基因型降低了非吸烟者中GCA的易感性（校正OR=0.72，95%CI=0.54-0.97），但p53 Arg72Pro对ESCC的易感性无影响。按p53 Arg72Pro基因型分层后，53BP1 T885G G/G基因型降低了携带Pro等位基因（Arg/Pro和Pro/Pro基因型）个体中GCA的易感性（校正OR=0.74，95%CI=0.57-0.95）。