Lu Mei, Zhang Rui Lan, Zhang Zheng Gang, Chopp Michael
Department of Biostatistics and Research Epidemiology, Henry Ford Hospital, One Ford Place, Ste. 3E, Detroit, MI 48202, USA.
J Neurosci Methods. 2008 Jan 30;167(2):376-83. doi: 10.1016/j.jneumeth.2007.08.015. Epub 2007 Aug 25.
Cell kinetics employed for embryonic models was modified and used to study the neuronogenesis in the subventricular zone (SVZ) in adult rats subjected to stroke. Enhanced analytical approaches were introduced and used to compare the cell cycle length (T(C)) and length in G(1) phase, T(G1), at various times after stroke to study the correlation between T(G1) and T(C) and to compare cell cycle evolution and proliferation profiles between the stroke and embryonic models. Our data indicate that cell cycle kinetics for the embryonic model can be applied to stroke in the adult. Significant reduction of T(G1) early after stroke (p<0.05) corresponds to an increase of neural progenitor cells remaining in the cycle at early times and cells exiting at later times. T(G1) correlates with T(C) (r=0.99, p<0.05). In conclusion, the analytical approaches proposed can be used to study the cell proliferation profiles in adult rats subjected to stroke with and without stroke therapy. The cell kinetics the cell proliferation profile differs between the stroke and embryonic models. T(C) evolution is three-fold slower in the cells and leave the cycle earlier and more frequently in the stroke model, compared to the embryonic model. T(C) is a surrogate measure of T(G1).
用于胚胎模型的细胞动力学方法经过修改后，被用于研究成年中风大鼠脑室下区（SVZ）的神经发生。引入了增强的分析方法，用于比较中风后不同时间点的细胞周期长度（T(C)）和G1期长度T(G1)，以研究T(G1)与T(C)之间的相关性，并比较中风模型和胚胎模型之间的细胞周期演变和增殖情况。我们的数据表明，胚胎模型的细胞周期动力学可应用于成年中风情况。中风后早期T(G1)显著缩短（p<0.05），这对应于早期留在细胞周期中的神经祖细胞数量增加，以及后期退出细胞周期的细胞数量增加。T(G1)与T(C)相关（r=0.99，p<0.05）。总之，所提出的分析方法可用于研究接受或未接受中风治疗的成年中风大鼠的细胞增殖情况。中风模型和胚胎模型的细胞动力学及细胞增殖情况有所不同。与胚胎模型相比，中风模型中细胞的T(C)演变速度慢三倍，且更早、更频繁地退出细胞周期。T(C)是T(G1)的替代指标。
