O'Donnell S R, Miller-Larsson A, Brattsand R, Rauchle K L
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
Agents Actions Suppl. 1991;34:491-5.
An animal model is described for testing the effects of anti-asthma drugs on mediator-induced airway inflammation. An in situ segment of trachea is slowly perfused with normal saline in spontaneously breathing, anaesthetised rats. Both the anti-asthma drug and the inflammatory mediator can be applied directly to the airway mucosal surface at the same and/or different times. The amount of plasma in samples of perfusate is monitored. Application of a mediator, such as bradykinin, results in an increase in the amount of plasma in the tracheal lumen (and hence in the perfusate). Plasma in the perfusate can be estimated without injecting a labelled tracer for plasma exudation by using a protein native fluorescence (PNF) method as described by Miller-Larsson & Brattsand (3).
描述了一种用于测试抗哮喘药物对介质诱导的气道炎症影响的动物模型。在自发呼吸的麻醉大鼠中，用生理盐水缓慢灌注气管的原位节段。抗哮喘药物和炎症介质都可以在相同和/或不同时间直接应用于气道黏膜表面。监测灌流液样本中的血浆量。应用介质（如缓激肽）会导致气管腔内（进而在灌流液中）血浆量增加。通过使用Miller-Larsson和Brattsand（3）所述的蛋白质天然荧光（PNF）方法，可以在不注射用于血浆渗出的标记示踪剂的情况下估计灌流液中的血浆。
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