Cavuşoğlu Cengiz, Karataş Eylem, Söyler Ilknur
Ege Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Bornova, Izmir.
Mikrobiyol Bul. 2007 Jul;41(3):385-93.
Molecular typing methods have greatly enhanced our understanding on epidemiology of tuberculosis and allowed us to identify outbreaks and intertransmission within populations. Recently, a set of 12 variable-number tandem repeat (VNTR), designated mycobacterial interspersed repetitive units (MIRU), has been described as being useful for the typing of M. tuberculosis. In this study, 26 rifampin (RIF) resistant M. tuberculosis isolates with known IS6110-RFLP patterns obtained from 26 different patients in Aegean Region were typed by MIRU-VNTR and the data were compared with IS6110-RFLP results. The results showed that in most isolates the clustering on the basis of IS6110 RFLP typing and that on the basis of MIRU-VNTR typing were in agreement. It was also determined that the loci including MIRU 16, MIRU 40, MIRU 26, MIRU 10, MIRU 04 and MIRU 31, respectively, have the highest allelic diversities and discriminatory power. In conclusion, since the discrimination level of conventional MIRU-VNTR including 12 loci might be variable, by the use of additional loci which present high degree of allelic differentiation, this method would be reliable for the epidemiologic studies.
分子分型方法极大地增进了我们对结核病流行病学的理解，并使我们能够识别群体内的疫情爆发和人际传播情况。最近，一组12个可变数目串联重复序列（VNTR），即分枝杆菌散布重复单位（MIRU），已被描述为对结核分枝杆菌分型有用。在本研究中，对从爱琴海地区26名不同患者中分离出的26株具有已知IS6110-RFLP模式的耐利福平（RIF）结核分枝杆菌菌株进行了MIRU-VNTR分型，并将数据与IS6110-RFLP结果进行了比较。结果表明，在大多数分离株中，基于IS6110 RFLP分型和基于MIRU-VNTR分型的聚类结果是一致的。还确定分别包括MIRU 16、MIRU 40、MIRU 26、MIRU 10、MIRU 04和MIRU 31的位点具有最高的等位基因多样性和鉴别力。总之，由于包括12个位点的传统MIRU-VNTR的鉴别水平可能存在差异，通过使用具有高度等位基因分化的额外位点，该方法对于流行病学研究将是可靠的。
