Prakasha B K, Ramakrishna Raju P, Karunasagar Indrani, Karunasagar Iddya
Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575 002, India.
Dis Aquat Organ. 2007 Aug 13;77(1):83-6. doi: 10.3354/dao01835.
Viral diseases have become a major constraint on the growth of shrimp aquaculture in India. During a study of new diseases in cultured tiger shrimp Penaeus monodon, diseased samples randomly collected from the southwestern and southeastern coasts of India were analyzed for white spot syndrome virus (WSSV), monodon baculovirus (MBV), and hepatopancreatic parvovirus (HPV) by nested PCR, and for Laem-Singh virus (LSNV) by reverse transcription PCR (RT-PCR). Of the 56 samples analyzed, 3 were positive for LSNV. These samples had signs of loose shell syndrome (LSS). Of the 3 samples that tested positive for LSNV, 2 were also positive for WSSV and MBV, and of these 2 samples, 1 was also positive for HPV. This is the first reported presence of LSNV in cultured shrimp in India.
病毒性疾病已成为印度对虾养殖业发展的主要制约因素。在一项对养殖斑节对虾新疾病的研究中,从印度西南和东南沿海随机采集的患病样本,通过巢式聚合酶链反应(PCR)分析了白斑综合征病毒(WSSV)、斑节对虾杆状病毒(MBV)和肝胰腺细小病毒(HPV),并通过逆转录聚合酶链反应(RT-PCR)分析了雷姆-辛格病毒(LSNV)。在分析的56个样本中,3个样本的LSNV呈阳性。这些样本有脱壳综合征(LSS)的症状。在LSNV检测呈阳性的3个样本中,2个样本的WSSV和MBV也呈阳性,在这2个样本中,1个样本的HPV也呈阳性。这是印度养殖对虾中首次报告存在LSNV。