通过聚合酶链式反应（PCR）检测孵化场养殖的斑节对虾后期幼体中的肝胰腺细小病毒（HPV）和其他病毒。

Detection by PCR of hepatopancreatic parvovirus (HPV) and other viruses in hatchery-reared Penaeus monodon postlarvae.

作者信息

Umesha Rudrappa K, Uma Arumugam, Otta Subhendu K, Karunasagar Indrani, Karunasagar Iddya

机构信息

Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India.

出版信息

Dis Aquat Organ. 2003 Dec 3;57(1-2):141-5. doi: 10.3354/dao057141.

DOI:10.3354/dao057141

PMID:14735932

Abstract

The prevalence of hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV) and white spot syndrome virus (WSSV) in samples of Penaeus monodon postlarvae (PL10 to PL20, 10 to 20 d old postlarvae) in India was studied by PCR. Samples collected from different hatcheries, and also samples submitted by farmers from different coastal states, were analyzed. HPV was detected in 34%) of the hatchery samples and 31% of the samples submitted by farmers, using a primer set designed for detection of HPV from P. monodon in Thailand. However, none of these samples were positive using primers designed for detection of HPV from P. chinensis in Korea. This indicated that HPV from India was more closely related to HPV from P. monodon in Thailand. MBV was detected in 64% of the samples submitted by the farmers and 71% of the hatchery samples. A total of 84 % of the samples submitted by farmers, and 91% of the hatchery samples, were found positive for WSSV. Prevalence of concurrent infections by HPV, MBV and WSSV was 27% in hatchery samples and 29%, in samples submitted by farmers. Only 8% of the hatchery samples and 16% of the samples submitted by farmers were negative for all 3 viruses. This is the first report on the prevalence of HPV in P. monodon postlarvae from India.

摘要

通过聚合酶链反应（PCR）研究了印度凡纳滨对虾后期幼体（PL10至PL20，即10至20日龄的后期幼体）样本中肝胰腺细小病毒（HPV）、对虾杆状病毒（MBV）和白斑综合征病毒（WSSV）的流行情况。对从不同孵化场采集的样本以及来自不同沿海邦的养殖户提交的样本进行了分析。使用为检测泰国凡纳滨对虾中的HPV而设计的引物组，在34%的孵化场样本和31%的养殖户提交的样本中检测到了HPV。然而，使用为检测韩国中国对虾中的HPV而设计的引物时，这些样本均未呈阳性。这表明印度的HPV与泰国凡纳滨对虾中的HPV关系更为密切。在养殖户提交的64%的样本和71%的孵化场样本中检测到了MBV。在养殖户提交的样本中有84%、在孵化场样本中有91%被发现WSSV呈阳性。HPV、MBV和WSSV并发感染在孵化场样本中的流行率为27%，在养殖户提交的样本中为29%。只有8%的孵化场样本和16%的养殖户提交的样本这三种病毒均呈阴性。这是关于印度凡纳滨对虾后期幼体中HPV流行情况的首次报告。