用于药物和核酸递送的定向进化

Directed evolution for drug and nucleic acid delivery.

作者信息

Hida Kaoru, Hanes Justin, Ostermeier Marc

机构信息

Department of Biomedical Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore MD, 21218, USA.

出版信息

Adv Drug Deliv Rev. 2007 Dec 22;59(15):1562-78. doi: 10.1016/j.addr.2007.08.022. Epub 2007 Aug 28.

DOI:10.1016/j.addr.2007.08.022

PMID:17933418

Abstract

Directed evolution is a term used to describe a variety of related techniques to rapidly evolve peptides and proteins into new forms that exhibit improved properties for specific applications. In this process, molecular biology techniques allow the creation of up to billions of mutants in a single experiment, which are then subjected to high-throughput screening to identify those with enhanced activity. Applications of directed evolution to drug and gene delivery have been recently described, including those that improve the effectiveness of therapeutic enzymes, targeting peptides and antibodies, and the effectiveness or tropism of viral vectors for use in gene therapy. This review first introduces fundamental concepts of directed evolution, and then discusses emerging applications in the field of drug and gene delivery.

摘要

定向进化是一个用于描述多种相关技术的术语，这些技术可将肽和蛋白质快速进化为新的形式，使其在特定应用中表现出改进的特性。在此过程中，分子生物学技术能够在单次实验中创造多达数十亿个突变体，然后对其进行高通量筛选，以鉴定出活性增强的突变体。最近已经报道了定向进化在药物和基因递送方面的应用，包括提高治疗性酶、靶向肽和抗体的有效性，以及改善用于基因治疗的病毒载体的有效性或趋向性。本文综述首先介绍定向进化的基本概念，然后讨论其在药物和基因递送领域的新兴应用。