不同条件下细粒棘球绦虫原头节的活力

Viability of Echinococcus granulosus protoscolices at different conditions.

作者信息

Diker Ali Ihsan, Tinar Recep, Senlik Bayram

机构信息

Uludag University, Faculty of Veterinary Medicine, Parasitology Department, Gorukle Campus, 16059 Bursa, Turkey.

出版信息

Vet Parasitol. 2007 Nov 30;150(1-2):84-7. doi: 10.1016/j.vetpar.2007.08.030. Epub 2007 Oct 22.

DOI:10.1016/j.vetpar.2007.08.030

PMID:17933466

Abstract

The aim of this study was to examine the longest survival time of Echinococcus granulosus protoscolices stored at different humidities and constant temperatures from -10 to +40 degrees C. Sheep livers containing hydatid cysts obtained from slaughterhouses were taken to the laboratory within 3h and transferred into incubation cabinet previously set at -10, 0, +10, +20, +30 and +40 degrees C with different relative humidity (RH). Viability of protoscolices was assessed by 0.1% eosin staining. The longest survival times were 3 days at -10 degrees C (50% RH), 36 days at 0 degrees C (60% RH), 28 days at 10 degrees C (65% RH), 12 days at 20 degrees C (70% RH), 4 days at 30 degrees C (75% RH) and 3 days at 40 degrees C (80% RH).

摘要

本研究的目的是检测细粒棘球绦虫原头节在-10至+40摄氏度不同湿度和恒定温度下储存的最长存活时间。从屠宰场获取的含有包虫囊肿的羊肝在3小时内被送往实验室，并转移到预先设置在-10、0、+10、+20、+30和+40摄氏度、具有不同相对湿度（RH）的培养箱中。通过0.1%伊红染色评估原头节的活力。最长存活时间分别为：-10摄氏度（50%RH）时为3天，0摄氏度（60%RH）时为36天，10摄氏度（65%RH）时为28天，20摄氏度（70%RH）时为12天，30摄氏度（75%RH）时为4天，40摄氏度（80%RH）时为3天。