Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz 71345-1731, Iran.
Exp Parasitol. 2011 Jan;127(1):14-7. doi: 10.1016/j.exppara.2010.06.021. Epub 2010 Jun 22.
Hydatid disease is one of the most important helminthic diseases worldwide. Hydatid cysts may be found anywhere in the body. The most effective treatment of hydatid cyst is surgical operation. Spillage of live protoscolices during the operation is the major cause of recurrence. Instillation of scolicidal agent into hydatid cyst is the most commonly employed measure to prevent this complication. To date, many scolicidal agents have been used for inactivation of the hydatid cyst content, however, most common scolicidal agents may cause unacceptable side-effects, limiting their use. In this study the scolicidal effect of warm water (45, 50, 55, and 60 °C) at different exposure times (1, 2, 3, 4, 5, 6, 8, 10, 12, and 15 min) is investigated. Protoscolices were collected aseptically from sheep livers containing hydatid cyst. Viability of protoscolices was determined by 0.1% eosin staining. Even though the highest scolicidal activity of warm water at 45 °C was 40.4% at the end of 15 min, the best scolicidal effect (100%) of warm water at 50, 55, and 60 °C was obtained after 5, 2, and 1 min, respectively. The results of this in vitro study showed that warm water at 50-60 °C can be regarded as an effective scolicidal agent. Warm water is commonly available, easily prepared, and inexpensive. In vivo scolicidal activity of warm water and also the possible side effects need further investigation.
包虫病是全球最重要的寄生虫病之一。包虫囊肿可在体内任何部位发现。包虫囊肿的最有效治疗方法是手术。术中活原头节溢出是复发的主要原因。向包虫囊肿中灌注杀囊剂是预防这种并发症最常用的措施。迄今为止，已经使用了许多杀囊剂来灭活包虫囊肿内容物，但是，大多数常见的杀囊剂可能会引起不可接受的副作用，限制了它们的使用。在这项研究中，研究了不同暴露时间（1、2、3、4、5、6、8、10、12 和 15 分钟）下 45、50、55 和 60°C 的温水的杀囊效果。原头节无菌地从含有包虫囊肿的绵羊肝脏中收集。通过 0.1%曙红染色来确定原头节的活力。尽管在 15 分钟结束时 45°C 温水的最高杀囊活性为 40.4%，但在 5、2 和 1 分钟时，50、55 和 60°C 温水获得了最佳的杀囊效果（100%）。体外研究的结果表明，50-60°C 的温水可视为有效的杀囊剂。温水普遍易得，易于制备且价格低廉。体内温水的杀囊活性以及可能的副作用需要进一步研究。
