c-Src是E3泛素连接酶Numb蛋白X1配体的PDZ相互作用伴侣和底物。
c-Src is a PDZ interaction partner and substrate of the E3 ubiquitin ligase Ligand-of-Numb protein X1.
作者信息
Weiss Andreas, Baumgartner Martin, Radziwill Gerald, Dennler Julia, Moelling Karin
机构信息
Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8006 Zurich, Switzerland.
出版信息
FEBS Lett. 2007 Oct 30;581(26):5131-6. doi: 10.1016/j.febslet.2007.09.062. Epub 2007 Oct 8.
The very C-terminus of c-Src is a ligand for PDZ domains. In a screen for PDZ domains that interact with c-Src, we identified one of the PDZ domains of the Ligand-of-Numb protein X1 (LNX1), a multiple PDZ domain scaffold and RING type E3 ubiquitin ligase. We demonstrate that the interaction of c-Src with LNX1 depends on the C-terminal PDZ ligand of c-Src. Furthermore, we show that c-Src phosphorylates LNX1. Moreover, c-Src itself is ubiquitinated by LNX1, suggesting an interdependent regulation of c-Src and LNX1.
c-Src的C末端是PDZ结构域的配体。在一项针对与c-Src相互作用的PDZ结构域的筛选中，我们鉴定出了Numb蛋白X1配体（LNX1）的一个PDZ结构域，LNX1是一种具有多个PDZ结构域的支架蛋白和RING型E3泛素连接酶。我们证明c-Src与LNX1的相互作用依赖于c-Src的C末端PDZ配体。此外，我们表明c-Src可使LNX1磷酸化。而且，c-Src自身被LNX1泛素化，这表明c-Src和LNX1之间存在相互依赖的调控关系。
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