El-Enshasy H A, Mohamed N A, Farid M A, El-Diwany A I
Bioprocess Development Department, GEBRI, Mubarak City for Scientific Research, New Burg Al Arab, 21934 Alexandria, Egypt.
Bioresour Technol. 2008 Jul;99(10):4263-8. doi: 10.1016/j.biortech.2007.08.050. Epub 2007 Oct 23.
In the present work, erythromycin production was carried out in submerged culture using Saccharopolyspora erythraea. Different experiments were conducted to optimize the cultivation medium through the change of carbon and nitrogen sources to cheaper one in order to reduce the cost of medium and to utilize sugar cane molasses as one of major sugar industry by-products in Egypt. It was found that the addition of sugar cane molasses a sole carbon source at a concentration of 60 g/l accompanied by corn steep liquor (as organic N-source) in combination with ammonium sulphate (as inorganic N-source) gave the maximal erythromycin production. The antibiotic production in this medium reached about 600 mg/l which is about 33% higher than the value obtained in glucose based medium. On the other hand, the addition of n-propanol in concentration of 1% (v/v) increased the antibiotic production reaching about 720 mg/l after 144 h. Concluding, the new medium formulation based on cheap carbon source, sugar cane molasses, was a good alternative solution for the production of erythromycin economically.
在本研究中，使用糖多孢红霉菌在深层培养中生产红霉素。进行了不同的实验，通过将碳源和氮源更换为更便宜的来源来优化培养基，以降低培养基成本，并利用甘蔗废蜜作为埃及主要制糖工业副产品之一。结果发现，添加浓度为60 g/l的甘蔗废蜜作为唯一碳源，同时添加玉米浆（作为有机氮源）和硫酸铵（作为无机氮源）时，红霉素产量最高。该培养基中的抗生素产量达到约600 mg/l，比在基于葡萄糖的培养基中获得的值高出约33%。另一方面，添加浓度为1%（v/v）的正丙醇可提高抗生素产量，144小时后达到约720 mg/l。总之，基于廉价碳源甘蔗废蜜的新培养基配方是经济生产红霉素的良好替代解决方案。
