Sánchez Julieta M, Turina Anahí del V, Perillo María A
Biofisica-Química, Depto. de Química, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Av. Velez Sarsfield 1611, X 5016GCA Córdoba, Argentina.
J Photochem Photobiol B. 2007 Nov 12;89(1):56-62. doi: 10.1016/j.jphotobiol.2007.08.004. Epub 2007 Sep 6.
In the present study we estimated the localization of ortho-nitrophenol (ONP) within model membranes through its efficiency to quench and to modify the anisotropy of DPH and TMA-DPH fluorescence. These fluorescent probes are known to sense the hydrocarbon core and the polar head group region of membranes, respectively. TMA-DPH fluorescence in MLVs was more efficiently quenched than DPH (K(q,TMA-DPH)=2.36 and K(q,DPH)=1.07 mM ns(-1)). Moreover, these results demonstrated the interfacial localization of ONP and may contribute to understand membrane-mediated mechanisms of ONP-induced toxicity and the behavior of ONP as a product of several enzymatic reactions occurring in the presence of lipid-water interfaces.
在本研究中，我们通过邻硝基苯酚（ONP）淬灭和改变二苯基己三烯（DPH）及三甲基胺 - 二苯基己三烯（TMA - DPH）荧光各向异性的效率，估算了其在模型膜中的定位。已知这些荧光探针分别可感知膜的烃核和极性头部基团区域。多层脂质体（MLVs）中TMA - DPH荧光比DPH更有效地被淬灭（K(q,TMA - DPH)=2.36且K(q,DPH)=1.07 mM ns(-1)）。此外，这些结果证明了ONP的界面定位，并可能有助于理解膜介导的ONP诱导毒性机制以及ONP作为在脂质 - 水界面存在时发生的几种酶促反应产物的行为。
