Rocco Anna, Fanali Salvatore
Institute of Chemical Methodologies, Consiglio Nazionale delle Ricerche, Area della Ricerca di Roma I, Via Salaria Km 29300, 00016 Monterotondo Scalo, Rome, Italy.
J Chromatogr A. 2008 May 16;1191(1-2):263-7. doi: 10.1016/j.chroma.2007.09.059. Epub 2007 Sep 29.
A fused silica capillary column was packed with RP(18) silica stationary phase entrapping the particles between two frits obtained by two different procedures. The inlet frit consisted of a short organic polymer made via a thermopolymerization process while the outlet frit was prepared by sintering the octadecylsilica (ODS) material. The packed column was employed in capillary electrochromatography (CEC) experiments for the separation of three selected test compounds. Retention time and separation efficiency were evaluated. Results were compared with those ones obtained with a packed capillary containing the same stationary phase entrapped between two sinterized frits. The novel packed column exhibited comparable separation efficiency and resolution with the traditional one. However, it allowed experiments without pressure support during the runs with no bubble formation.
一根熔融石英毛细管柱填充了RP(18)硅胶固定相，通过两种不同方法获得的两个筛板将颗粒夹在中间。入口筛板由通过热聚合过程制成的短有机聚合物组成，而出口筛板则通过烧结十八烷基硅胶（ODS）材料制备。填充柱用于毛细管电色谱（CEC）实验，以分离三种选定的测试化合物。评估了保留时间和分离效率。将结果与在两个烧结筛板之间夹有相同固定相的填充毛细管获得的结果进行了比较。新型填充柱与传统填充柱具有相当的分离效率和分辨率。然而，它允许在运行过程中无需压力支持且不形成气泡的情况下进行实验。
