Kagramanova A S, Kapelinskaia T V, Korolev A L, Mukha D V
Mol Biol (Mosk). 2007 Jul-Aug;41(4):608-15.
This is the first report providing results on identification, cloning, and sequencing of extended fragments (5'-truncated copies) of R1 and R2 retrotransposons integrated into Blattella germanica genome. Comparative structural analysis of the received clones revealed two distinct subfamilies of R1 elements. However, all B. germanica R1 clones have two common features: poly(T) tails and similar target site duplications. Nucleotide structure and organization of five sequenced R2 fragments was similar. Analysis of R2 nucleotide sequences revealed typical deletions at the 3'end of target sites and lack of homopolynucleotides tails.
这是首份关于整合到德国小蠊基因组中的R1和R2逆转座子延伸片段(5'-截短拷贝)的鉴定、克隆及测序结果的报告。对所获克隆的比较结构分析揭示了R1元件的两个不同亚家族。然而,所有德国小蠊R1克隆都有两个共同特征:聚(T)尾和相似的靶位点重复序列。五个测序的R2片段的核苷酸结构和组织相似。对R2核苷酸序列的分析揭示了靶位点3'端典型的缺失以及同聚核苷酸尾的缺失。