[德国小蠊浓核病毒：检测、核苷酸序列及基因组结构]
[A densovirus of German cockroach Blatella germanica: detection, nucleotide sequence and genome organization].
作者信息
Mukha D V, Schal K
机构信息
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.
出版信息
Mol Biol (Mosk). 2003 Jul-Aug;37(4):607-18.
A new Blattella germanica densovirus (BgDNV, Parvoviridae: Densovirinae, Densovirus) was found. Virus DNA and cockroach tissues infected with BgDNV were examined by electron microscopy. Virus particles about 20 nm in diameter were observed both in the nucleus and in the cytoplasm of infected cells. Virus DNA proved to be a linear molecule sized about 1.2 microns. The complete BgDNV genome was sequenced and analyzed. Five ORF were detected: two coded for structural capsid proteins and were on one DNA strand, and three coded for regulatory proteins and were on the other strand. Potential promoters and polyadenylation signals were identified. Structural analysis was performed for terminal inverted repeats containing extended palindromes. The genome structure of BgDNV was compared with that of other Parvoviridae.
发现了一种新的德国小蠊浓核病毒（BgDNV，细小病毒科：浓核病毒亚科，浓病毒属）。通过电子显微镜检查了感染BgDNV的病毒DNA和蟑螂组织。在受感染细胞的细胞核和细胞质中均观察到直径约20纳米的病毒颗粒。病毒DNA被证明是一个大小约为1.2微米的线性分子。对完整的BgDNV基因组进行了测序和分析。检测到五个开放阅读框：两个编码结构衣壳蛋白，位于一条DNA链上，另外三个编码调节蛋白，位于另一条链上。鉴定出了潜在的启动子和聚腺苷酸化信号。对含有延伸回文序列的末端反向重复序列进行了结构分析。将BgDNV的基因组结构与其他细小病毒科的基因组结构进行了比较。
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