粒细胞通过与树突状细胞直接相互作用而被激活。
Activation of granulocytes by direct interaction with dendritic cells.
作者信息
Tsuda M, Inaba M, Sakaguchi Y, Fukui J, Ueda Y, Omae M, Ando Y, Mukaide H, Guo K, Yabuki T, Nakatani T, Ikehara S
机构信息
First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan.
出版信息
Clin Exp Immunol. 2007 Nov;150(2):322-31. doi: 10.1111/j.1365-2249.2007.03490.x.
Granulocytes from human peripheral blood were co-cultured with conventional dendritic cells (cDC) or plasmacytoid DCs (pDC) to examine the effects of DCs on the activation or function of granulocytes. After co-culture of granulocytes with DCs, expression of the activation markers of granulocytes (CD63 and CD64) was up-regulated, and increased expression of CD50, the activation marker and ligand for CD209 (DC-SIGN) was also observed. The interaction of granulocytes with DCs was visualized as the cluster where DCs, especially cDCs, were surrounded by granulocytes to form a 'rosette'. After co-culture of granulocytes with cDCs, the secretion of elastase from granulocytes was enhanced significantly when examined cytohistochemically and by enzyme-linked immunosorbent assay. An increase in myeloperoxidase (another activation index of granulocytes) was also observed after co-culture with DCs. These findings suggest the functional and phenotypical activation of granulocytes by interaction with DCs. Furthermore, we examined the involvement of adhesion molecules in the granulocyte-DC interaction, and found that CD209 participates to some extent in this interaction.
将人外周血中的粒细胞与常规树突状细胞（cDC）或浆细胞样树突状细胞（pDC）共培养，以研究树突状细胞对粒细胞激活或功能的影响。粒细胞与树突状细胞共培养后，粒细胞激活标志物（CD63和CD64）的表达上调，同时还观察到CD50（CD209（DC-SIGN）的激活标志物和配体）的表达增加。粒细胞与树突状细胞的相互作用表现为树突状细胞（尤其是cDC）被粒细胞包围形成“玫瑰花结”的聚集。粒细胞与cDC共培养后，通过细胞组织化学和酶联免疫吸附测定法检测发现，粒细胞中弹性蛋白酶的分泌显著增强。与树突状细胞共培养后，还观察到髓过氧化物酶（粒细胞的另一个激活指标）增加。这些发现表明粒细胞通过与树突状细胞相互作用实现功能和表型激活。此外，我们研究了黏附分子在粒细胞与树突状细胞相互作用中的作用，发现CD209在一定程度上参与了这种相互作用。
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