Salazar I, Yllera M M
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, University of Santiago, Lugo, Spain.
Funct Dev Morphol. 1991;1(1):59-63.
We describe the chronology of the development of the renal system and gonads of 19 greyhound foetuses. The smallest specimens were embedded in paraffin wax and were sectioned serially at 10 microns; the rest were sectioned at 50 microns in an LKB 2250 PMV cryomicrotome. Selected sections were stained with haematoxylin and eosin. The mesonephros, metanephros and gonads were measured in drawings of the sections, using the area fraction programme of a Vids II image analyse.
我们描述了19只灵缇胎儿肾脏系统和性腺的发育时间顺序。最小的标本用石蜡包埋,以10微米的厚度连续切片;其余标本在LKB 2250 PMV冷冻切片机上以50微米的厚度切片。选取的切片用苏木精和伊红染色。利用Vids II图像分析的面积分数程序,在切片图中对中肾、后肾和性腺进行测量。