Friebová-Zemanová Z, Schakhmatova J, Szarska E, Kopecký J, Houstĕk J, Drahota Z
Physiol Bohemoslov. 1987;36(2):125-34.
The protein spectra of two fractions (the soluble and the membrane fraction) of chick embryo kidney homogenates were isolated by electrophoresis on polyacrylamide gels with the aim of detecting the kidney differentiation process at the molecular level and, at the same time, of evaluating similarities in the construction of the mesonephros and metanephros at this level. Corresponding stages of the above two types of kidney were chosen for studying changes in protein structure during differentiation--i.e. the outset of differentiation (the 6-day mesonephros, the 11-day metanephros) and the stage of full maturity (the 14-day mesonephros, the 20-day metanephros). A total of 36 proteins was distinguished. The analysis of the protein spectra showed that the number of proteins changes but slightly during differentiation; the protein composition of the two types of kidney during differentiation altered by 20-35% of the total number of proteins; the similarity of the protein composition of the corresponding stages of mesonephros and metanephros, expressed as the proportion of the number of identical proteins, was greater than the mutual similarity of different developmental stages of the same type of kidney. The percentage of different proteins at corresponding stages of the kidneys varied from 5% to 23% of the total number of proteins detected.
通过在聚丙烯酰胺凝胶上进行电泳，分离鸡胚肾匀浆的两个组分（可溶性组分和膜组分）的蛋白质谱，目的是在分子水平上检测肾分化过程，同时评估中肾和后肾在此水平上结构的相似性。选择上述两种类型肾脏的相应阶段来研究分化过程中蛋白质结构的变化，即分化开始阶段（6日龄中肾，11日龄后肾）和完全成熟阶段（14日龄中肾，20日龄后肾）。共区分出36种蛋白质。蛋白质谱分析表明，分化过程中蛋白质数量变化不大；两种类型肾脏在分化过程中的蛋白质组成变化占蛋白质总数的20%-35%；中肾和后肾相应阶段蛋白质组成的相似性，以相同蛋白质数量的比例表示，大于同一类型肾脏不同发育阶段之间的相互相似性。肾脏相应阶段不同蛋白质的百分比占检测到的蛋白质总数的5%至23%。
