创伤性脑损伤（TBI）后内源性血管生成抑制剂内皮抑素/ XVIII型胶原蛋白的损伤部位表达

Lesional expression of the endogenous angiogenesis inhibitor endostatin/collagen XVIII following traumatic brain injury (TBI).

作者信息

Mueller C A, Schluesener H J, Fauser U, Conrad S, Schwab J M

机构信息

Institute of Brain Research, Medical School, Eberhard-Karls University Tuebingen, Calwerstr. 3, 72076 Tuebingen, Germany.

出版信息

Exp Neurol. 2007 Dec;208(2):228-37. doi: 10.1016/j.expneurol.2007.07.020. Epub 2007 Aug 21.

DOI:10.1016/j.expneurol.2007.07.020

PMID:17942095

Abstract

We have analysed the expression of the endogenous angiogenesis inhibitor endostatin/collagen XVIII following stab wound injury and observed a highly significant (p<0.0001) lesional accumulation confined to areas of pan-necrotic injury and developing secondary damage. Maximal cell numbers were detected at Day 14, declining until Day 21 after injury. Further, endostatin/collagen XVIII(+) monocytic cells accumulated in Virchow-Robin spaces where they formed cell clusters. Besides being prevailingly localised to ED1(+) activated microglia/macrophages, endostatin/collagen XVIII expression was also detected by subendothelial surrounding vessels in the lesioned area. Late and prolonged accumulation of endostatin/collagen XVIII(+) microglia/macrophages and increased numbers of endostatin/collagen XVIII(+) subendothelial cells/vessels in areas of vascular pruning and regression, point to a role in the termination of the transient angiogenic response, linked to a "late" inflammatory milieu.

摘要

我们分析了刺伤损伤后内源性血管生成抑制剂内皮抑素/ XVIII型胶原蛋白的表达，观察到其在全坏死损伤区域及继发损伤区域有高度显著（p<0.0001）的损伤部位蓄积。在损伤后第14天检测到最大细胞数，之后直至第21天逐渐下降。此外，内皮抑素/ XVIII型胶原蛋白阳性单核细胞在血管周围间隙蓄积并形成细胞簇。除了主要定位于ED1阳性活化小胶质细胞/巨噬细胞外，在损伤区域的血管周围内皮细胞下也检测到内皮抑素/ XVIII型胶原蛋白的表达。在血管修剪和消退区域，内皮抑素/ XVIII型胶原蛋白阳性小胶质细胞/巨噬细胞的晚期和长期蓄积以及内皮抑素/ XVIII型胶原蛋白阳性内皮细胞/血管数量增加，表明其在与“晚期”炎症环境相关的短暂血管生成反应终止中发挥作用。