通过固相片段缩合合成硫代磷酸酯寡核苷酸-肽缀合物。

Synthesis of phosphorothioate oligonucleotide-peptide conjugates by solid phase fragment condensation.

作者信息

Diala Irmina, Osada Akira, Maruoka Shinji, Imanisi Takashi, Murao Satoshi, Ato Taishi, Ohba Hideki, Fujii Masayuki

机构信息

School of Humanity Oriented Science and Technology, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.

出版信息

Bioorg Med Chem Lett. 2007 Dec 1;17(23):6576-8. doi: 10.1016/j.bmcl.2007.09.101. Epub 2007 Oct 5.

DOI:10.1016/j.bmcl.2007.09.101

PMID:17942304

Abstract

Phosphorothioate oligonucleotide-peptide conjugates were synthesized by solid phase fragment condensation (SPFC). Arginine rich peptides could be successfully conjugated in 2.8-13.4% isolated yields. All the products were fully characterized by reversed phase HPLC and MALDI-TOF-MS to give satisfactory results.

摘要

硫代磷酸酯寡核苷酸 - 肽缀合物通过固相片段缩合（SPFC）合成。富含精氨酸的肽可以以2.8 - 13.4%的分离产率成功缀合。所有产物均通过反相高效液相色谱（HPLC）和基质辅助激光解吸电离飞行时间质谱（MALDI - TOF - MS）进行了全面表征，结果令人满意。