Stevens Charles F, Williams James H
Molecular Neurobiology Lab, The Salk Institute, La Jolla, CA 92037, USA.
J Neurophysiol. 2007 Dec;98(6):3221-9. doi: 10.1152/jn.00857.2007. Epub 2007 Oct 17.
A readily releasable pool (RRP) of synaptic vesicles has been identified at hippocampal synapses with application of hypertonic solution. RRP size correlates with important properties of synaptic function such as release probability. However, a discrepancy in RRP size has been reported depending on the method used to evoke synaptic release. This study was undertaken to determine quantitative relationships between the RRP defined with hypertonic solution and that released with trains of action potentials. We find that asynchronous release at cell culture synapses contributes significantly to the discharge of the RRP with trains of action potentials and that RRP size is the same when elicited by either nerve stimuli or hypertonic challenge.
通过应用高渗溶液,已在海马突触中鉴定出一个易于释放的突触小泡池(RRP)。RRP的大小与突触功能的重要特性(如释放概率)相关。然而,根据用于诱发突触释放的方法,已报道RRP大小存在差异。本研究旨在确定用高渗溶液定义的RRP与通过一系列动作电位释放的RRP之间的定量关系。我们发现,细胞培养突触处的异步释放在通过一系列动作电位使RRP放电中起重要作用,并且当由神经刺激或高渗刺激引发时,RRP大小相同。