Celis J E, Leffers H, Rasmussen H H, Madsen P, Honoré B, Gesser B, Dejgaard K, Olsen E, Ratz G P, Lauridsen J B
Institute of Medical Biochemistry, Aarhus University, Denmark.
Electrophoresis. 1991 Nov;12(11):765-801. doi: 10.1002/elps.1150121103.
The master two-dimensional gel database of human AMA cells currently lists 3801 cellular and secreted proteins, of which 371 cellular polypeptides (306 IEF; 65 NEPHGE) were added to the master images during the last 10 months. These include: (i) very basic and acidic proteins that do not focus under normal running conditions and (ii) low-abundant proteins that can only be detected after prolonged gel exposure. Annotation categories updated in this version include "protein name", "antibody against protein", "cellular localization", and "microsequenced proteins". New entries include "human autoantigens" and "cDNAs". For convenience we have included an alphabetical list of all known proteins recorded in this database. In the long run, the main goal of this database is to link protein and DNA sequencing and mapping information (Human Genome Program) and to provide an integrated picture of the expression levels and properties of the thousands of proteins that orchestrate various cellular functions both under physiological and abnormal conditions.
人类AMA细胞的主二维凝胶数据库目前列出了3801种细胞和分泌蛋白，其中在过去10个月里有371种细胞多肽（306种IEF；65种NEPHGE）被添加到主图像中。这些包括：（i）在正常运行条件下不聚焦的非常碱性和酸性的蛋白质，以及（ii）只有在长时间凝胶曝光后才能检测到的低丰度蛋白质。此版本中更新的注释类别包括“蛋白质名称”、“抗蛋白质抗体”、“细胞定位”和“微测序蛋白质”。新条目包括“人类自身抗原”和“cDNA”。为方便起见，我们提供了该数据库中记录的所有已知蛋白质的字母列表。从长远来看，该数据库的主要目标是将蛋白质和DNA测序及图谱信息（人类基因组计划）联系起来，并提供在生理和异常条件下协调各种细胞功能的数千种蛋白质的表达水平和特性的综合图景。
