Bogusiewicz Michał, Stryjecka-Zimmer Marta, Postawski Krzysztof, Jakimiuk Artur J, Rechberger Tomasz
Second Department of Gynecology, Medical University of Lublin, Lubin, Poland.
Gynecol Endocrinol. 2007 Sep;23(9):541-6. doi: 10.1080/09513590701557416.
Matrix metalloproteinase-2 and -9 (MMP-2 and -9) are proteolytic enzymes degrading extracellular matrix proteins, mainly collagen type IV. Recent reports show that these proteases may be implicated in the growth of uterine leiomyoma. The aim of the present study was to evaluate the activity of MMP-2 and MMP-9, the contents of their tissue inhibitors (TIMP-1 and TIMP-2) and the immunolocalization of collagen type IV in uterine leiomyoma and corresponding myometrium.
Material for the study comprised specimens of uterine leiomyomas and corresponding myometrium derived from 20 hysterectomized women. The activity of MMP-2 and MMP-9 in tissue extracts was evaluated by semi-quantitative zymography. TIMPs were measured by enzyme-linked inmmunosorbent assay. Protein immunohistochemistry was applied for detection of collagen type IV.
Activity and activation ratio of MMP-2 were significantly higher in leiomyomas than myometrium. The activity of MMP-9 was weak and did not differ between the investigated tissues. Contents of TIPM-1 and TIPM-2 were similar in both tissues. In both leiomyomas and myometrium, collagen type IV was localized in the extracellular matrix embedding bundles of smooth muscle cells, but was absent in areas of extracellular matrix accumulation within leiomyomas and in larger septa separating muscle fibers in normal myometrium.
MMP-2 may be implicated in pathogenesis of leiomyoma.
基质金属蛋白酶-2和-9（MMP-2和MMP-9）是降解细胞外基质蛋白（主要是IV型胶原）的蛋白水解酶。最近的报道表明，这些蛋白酶可能与子宫平滑肌瘤的生长有关。本研究的目的是评估子宫平滑肌瘤及相应子宫肌层中MMP-2和MMP-9的活性、其组织抑制剂（TIMP-1和TIMP-2）的含量以及IV型胶原的免疫定位。
研究材料包括来自20例接受子宫切除术妇女的子宫平滑肌瘤标本及相应子宫肌层。通过半定量酶谱法评估组织提取物中MMP-2和MMP-9的活性。通过酶联免疫吸附测定法检测TIMP。应用蛋白质免疫组织化学法检测IV型胶原。
平滑肌瘤中MMP-2的活性和活化率显著高于子宫肌层。MMP-9的活性较弱，在所研究的组织之间无差异。两种组织中TIMP-1和TIMP-2的含量相似。在平滑肌瘤和子宫肌层中，IV型胶原均定位于包绕平滑肌细胞束的细胞外基质中，但在平滑肌瘤内细胞外基质积聚区域以及正常子宫肌层中分隔肌纤维的较大间隔中不存在。
MMP-2可能与平滑肌瘤的发病机制有关。
