[滇黄芩快速繁殖研究]
[Study on rapid reproduction of Scutellaria amoena].
作者信息
Li Ya-qiong, You Chun, Yang Guan
机构信息
Yunnan College of TCM, Kunming 650200, China.
出版信息
Zhong Yao Cai. 2007 Jul;30(7):761-2.
PMID:17944178
Abstract
Tissue cultures of Scutellaria amoena were established from the young stem explants on MS + IAA 0.1 mg/L + BA 0. 5 (0.2) mg/L. Propagation cultures were established on MS + BA 0.2 (1.5) mg/L + IAA 0.1 mg/L + NAA 0.1 mg/L + 2, 4-D 0.5 (1.5) mg/L. Rooting of planlet was established on 1/2 MS + NAA 0.1 (0.2) mg/L + IBA 0.1 (0.2) mg/L + PP333 1.0 mg/L and the rate can reach 100%.
摘要
从滇黄芩幼嫩茎段外植体在添加了0.1毫克/升吲哚乙酸（IAA）和0.5（0.2）毫克/升苄氨基腺嘌呤（BA）的MS培养基上建立了组织培养体系。增殖培养是在添加了0.2（1.5）毫克/升BA、0.1毫克/升IAA、0.1毫克/升萘乙酸（NAA）和0.5（1.5）毫克/升2,4-二氯苯氧乙酸（2,4-D）的MS培养基上进行的。在添加了0.1（0.2）毫克/升NAA、0.1（0.2）毫克/升吲哚丁酸（IBA）和1.0毫克/升多效唑（PP333）的1/2MS培养基上进行植株生根培养，生根率可达100%。
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