白术组织培养与快速繁殖研究

[Study on tissue culture and rapid propagation of Atractylodes macrocephala].

作者信息

Zhu Yu-Qiu, Xia Guo-Hua, Fang Hui-Gang, Fu Shun-Hua, He Fu-Ji

机构信息

Faculty of Forestry and Biotechnology, Zhejiang Forestry University, Lin'an, Zhejiang 311300, China.

出版信息

Zhong Yao Cai. 2006 Mar;29(3):212-3.

PMID:16850713

Abstract

A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz.. The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation. MS + BA 1.0 mg/L + NAA 0.3 mg/L + GA3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also. MS + BA 3.0 mg/L + IBA 0.5 mg/L was suitable for the proliferation of axillary buds, with an induction rate of 95%. 1/2MS + IBA 0.1 - 0.5 mg/L was optimum for rooting, with a rooting percentage more than 90%. The survival rate of transplanted plantlets was more than 90%.

摘要

以白术为外植体进行了组织培养研究。结果表明，NAA是叶片和叶柄诱导愈伤组织以及芽分化的主导因素。MS + BA 1.0毫克/升 + NAA 0.3毫克/升 + GA3 0.2毫克/升是叶片和叶柄诱导愈伤组织以及芽分化的最佳培养基。MS + BA 3.0毫克/升 + IBA 0.5毫克/升适合腋芽增殖，诱导率为95%。1/2MS + IBA 0.1 - 0.5毫克/升是生根的最佳培养基，生根率超过90%。移栽苗的成活率超过90%。