[一种通过流式细胞术研究斑蝥素水疱成分的技术]
[A technique for investigating the component of cantharidin blisters by flow cytometry].
作者信息
Zou Xiao-jun, Liu Hui-ping, Cheng Tao, Shi Li, Zu Ping, Zhang Yi-jun
机构信息
Department of Infectious Diseases, Communicable Diseases Center of PLA, The 458th Hospital of PLA, Guangzhou 510602, China.
出版信息
Zhong Yao Cai. 2007 Jul;30(7):823-5.
OBJECTIVE
Cantharidimide cause blister. The effect of blister on immunoregulation was investigated.
METHODS
Cantharidimide was placed on the skin, 48h later, the blister was analyzed by flow cytometry.
RESULTS
The blister contained 1 x 10(6) - 1 x 10(7) cells per ml, most of which were neutrophils, macrophages, dendritic cells (DC), and IL-12 secreted by Thl cells.
CONCLUSION
There are high concent of DC in the blister, which is differential and induce the secretion of Th1, the activation of T cell. The blister modulate the biological response of patients and is helpful for treatment with infective disease.
目的
斑蝥素可引起水疱。研究水疱对免疫调节的作用。
方法
将斑蝥素置于皮肤上，48小时后，通过流式细胞术分析水疱。
结果
水疱每毫升含有1×10⁶ - 1×10⁷个细胞，其中大多数是中性粒细胞、巨噬细胞、树突状细胞（DC）以及Th1细胞分泌的IL - 12。
结论
水疱中存在高浓度的DC，具有差异性并诱导Th1分泌，激活T细胞。水疱调节患者的生物学反应，有助于感染性疾病的治疗。
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