用斑蝥素治疗的患皮肤利什曼病的BALB/c小鼠中γ干扰素和白细胞介素-4模式
Interferon-gamma and interlukin-4 patterns in BALB/c mice suffering from cutaneous leishmaniasis treated with cantharidin.
作者信息
Maroufi Yahya, Ghaffarifar Fatemeh, Dalimi Abdolhosein, Sharifi Zohreh
机构信息
Department of Parasitology, Faculty of Medical Sciences, Zabol University of Medical Sciences, Zabol, IR Iran.
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.
出版信息
Jundishapur J Microbiol. 2014 Jun;7(6):e10907. doi: 10.5812/jjm.10907. Epub 2014 Jun 1.
BACKGROUND
Cutaneous leishmaniasis is a health problem in the world. Lesions should be treated on cosmetically or functionally important sites, such as the face and hands. Cantharidin is a terpenoid compound produced naturally by beetles of Meloidae and Oedemeridae families.
OBJECTIVES
The current study aimed to investigate the effect of cantharidin on Cutaneous Leishmaniasis (CL) lesions and IFN-γ and IL-4 patterns in infected BALB/c mice.
MATERIALS AND METHODS
INFECTED BALB/C MICE WERE DIVIDED INTO FIVE GROUPS AS: untreated (control group), eucerin-treated and 0.05%, 0.1% and 0.5% cantharidin-treated. Lesions diameter was measured by Vernier caliper every three days for four weeks. Cytokines levels were measured by enzyme-linked immunosorbent assay (ELISA) using U-CyTech kit.
RESULTS
The results indicated that treatment with cantharidin exacerbates lesions compared with the controls, except for 0.05% cantharidin dose that restrained lesion growth significantly. Interferon gamma level in cantharidin-treated groups was significantly less than that of the control group. But interlukin-4 level was similar among the groups.
CONCLUSIONS
The current study results indicated that high doses of cantharidin exacerbates leishmaniasis lesion, but low dose of cantharidin inhibits lesion growth.
背景
皮肤利什曼病是一个全球性的健康问题。对于面部和手部等在美容或功能上较为重要的部位的病变应进行治疗。斑蝥素是芫菁科和拟天牛科甲虫自然产生的一种萜类化合物。
目的
本研究旨在探讨斑蝥素对感染的BALB/c小鼠皮肤利什曼病（CL）病变以及IFN-γ和IL-4模式的影响。
材料和方法
将感染的BALB/C小鼠分为五组：未治疗组（对照组）、优色林治疗组以及0.05%、0.1%和0.5%斑蝥素治疗组。使用游标卡尺每三天测量一次病变直径，持续四周。使用U-CyTech试剂盒通过酶联免疫吸附测定（ELISA）测量细胞因子水平。
结果
结果表明，与对照组相比，除0.05%斑蝥素剂量能显著抑制病变生长外，斑蝥素治疗会使病变加重。斑蝥素治疗组的干扰素γ水平显著低于对照组。但各组间白细胞介素-4水平相似。
结论
本研究结果表明，高剂量斑蝥素会加重利什曼病病变，但低剂量斑蝥素可抑制病变生长。
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