[Study on the expression of suppressor of cytokine signaling-1 in liver and spleen of septic mice].

OBJECTIVE

To investigate the change in suppressor of cytokine signaling-1 (SOCS1) gene in the liver and the spleen of septic mouse, and to find out its probable mechanisms of action in sepsis.

METHODS

Cecal ligation and puncture (CLP) was adopted to reproduce sepsis model. The liver and the spleen tissues were harvested and RNA and protein were respectively extracted. The contents of the regulatory genes SOCS1 mRNA were determined by reverse transcription-polymerase chain reaction (RT-PCR) and regulatory content of protein was detected by Western blotting. The SPSS statistics software was adopted to calculate the correlation.

RESULTS

The expressions of SOCS1 on gene and protein in the liver were markedly upregulated at 6 th hour. The gene expression peaked at the 24 th hour (P<0.05).The expression of protein was persistently high. However, the expression of SOCS1 was only detected in the spleen, and it obviously rose in strength with the passage of time, and it remained in a high level. By statistical analysis, positive correlations were found between the gene and protein expressions of SOCS1 (y=0.110+/-5.765 x 10(-3) x, r=0.837, F=93.309, P<0.01).

CONCLUSION

CLP induced sepsis can induce the up-regulation of the expressions of SOCS1, indicating that SOCS1 play important role in the change in immune system in sepsis. They may be used to intervene sepsis so as to improve the outcome of sepsis.