Maier R, Schmid P, Cox D, Bilbe G, McMaster G K
Ciba-Geigy Ltd., Biotechnology Department, Basel, Switzerland.
Mol Cell Endocrinol. 1991 Dec;82(2-3):191-8. doi: 10.1016/0303-7207(91)90031-m.
We have studied the expression of transforming growth factor (TGF)-beta 1, -beta 2, and -beta 3 in the non-lactating and lactating bovine mammary gland by in situ hybridization. All three isoforms were expressed in the lobuloalveolar framework of the non-lactating and lactating gland although marked differences were apparent in their spatial distribution. TGF-beta 1 was expressed predominantly by the epithelial cells of the lobules although expression was also observed in the intralobular stroma cells lining the epithelium. In contrast, TGF-beta 2 expression was only observed in the epithelial cells. TGF-beta 3 transcripts were expressed at the highest levels and were observed in almost all cells of the lobule. No TGF-beta signals were found in the interlobular regions of the mammary gland. The possible regulatory functions of these molecules in development of the mammary gland and on differentiation processes in the neonate are discussed.
我们通过原位杂交研究了转化生长因子（TGF）-β1、-β2和-β3在非泌乳期和泌乳期奶牛乳腺中的表达。所有三种异构体在非泌乳期和泌乳期腺体的小叶腺泡结构中均有表达，尽管它们的空间分布存在明显差异。TGF-β1主要由小叶的上皮细胞表达，尽管在上皮内衬的小叶内基质细胞中也观察到表达。相比之下，TGF-β2仅在上皮细胞中观察到表达。TGF-β3转录本表达水平最高，在小叶的几乎所有细胞中都能观察到。在乳腺的小叶间区域未发现TGF-β信号。讨论了这些分子在乳腺发育和新生儿分化过程中的可能调节功能。
