Christianson Julie A, Gebhart Gerald F
Department of Anesthesiology, Center for Pain Research, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA.
Nat Protoc. 2007;2(10):2624-31. doi: 10.1038/nprot.2007.392.
Colorectal distension (CRD) is a widely used and reliable method for evaluating colon sensitivity in unanesthetized animals, including humans. Hollow organ distension is a mechanical stimulus that replicates in humans the sensation and pattern of referral of their visceral pain. In animals, CRD has been employed to evaluate drug efficacy, strain, sex or genetic differences and changes in colon sensitivity after inflammation or irritation of the distal colon. Responses to CRD are measured as electromyographic (EMG) recordings of the abdominal musculature, termed the visceromotor response. This protocol will provide sufficient detail to allow an investigator to surgically prepare a mouse for CRD, construct distending balloons, distend the colon, and accumulate and analyze data from EMG recordings; examples are also provided to illustrate typical experimental outcomes. CRD recording sessions are typically 2 h in duration.
结肠扩张（CRD）是一种广泛应用且可靠的方法，用于评估包括人类在内的未麻醉动物的结肠敏感性。中空器官扩张是一种机械刺激，可在人类中复制其内脏疼痛的感觉和牵涉模式。在动物中，CRD已被用于评估药物疗效、品系、性别或基因差异以及远端结肠炎症或刺激后结肠敏感性的变化。对CRD的反应通过腹部肌肉组织的肌电图（EMG）记录来测量，称为内脏运动反应。本方案将提供足够详细的信息，使研究人员能够为小鼠进行CRD手术准备、构建扩张球囊、扩张结肠，并收集和分析EMG记录的数据；还提供了示例来说明典型的实验结果。CRD记录 sessions通常持续2小时。
