牛奥斯特他线虫对伊维菌素耐药性的实验性选择。
Experimental selection for ivermectin resistance in Ostertagia ostertagi in cattle.
作者信息
Van Zeveren A M, Casaert S, Alvinerie M, Geldhof P, Claerebout E, Vercruysse J
机构信息
Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
出版信息
Vet Parasitol. 2007 Nov 30;150(1-2):104-10. doi: 10.1016/j.vetpar.2007.09.004. Epub 2007 Oct 18.
Recent reports of suspected ivermectin (IVM) resistance in Ostertagia ostertagi have highlighted the need for research into the mechanisms of IVM resistance. However, there are no reports of resistant field isolates of O. ostertagi, which have been characterized for molecular research. Therefore, an anthelmintic susceptible O. ostertagi population was selected for IVM resistance by repeatedly exposing the population to subtherapeutic and therapeutic levels of IVM over 10 generations. In each selection round, a group of calves was infected with the progeny of the previous IVM-selected O. ostertagi population. In the last selection round a therapeutic IVM dose (0.2 mg/kg BW) only reduced the faecal egg counts by 57% and 65% on days 7 and 14 after treatment, respectively. In contrast, the therapeutic IVM dose was 100% effective at eliminating the parental IVM-susceptible isolate.
近期关于奥斯特他线虫对伊维菌素（IVM）产生耐药性的疑似报道凸显了对IVM耐药机制进行研究的必要性。然而，目前尚无已被鉴定用于分子研究的奥斯特他线虫耐药性野外分离株的报道。因此，通过将一个对驱虫药敏感的奥斯特他线虫群体连续10代暴露于亚治疗剂量和治疗剂量的IVM中，筛选出对IVM具有耐药性的群体。在每一轮筛选中，一组犊牛感染上一轮经IVM筛选的奥斯特他线虫群体的后代。在最后一轮筛选中，治疗剂量的IVM（0.2 mg/kg体重）在治疗后第7天和第14天仅分别使粪便虫卵计数降低了57%和65%。相比之下，治疗剂量的IVM在清除亲本IVM敏感分离株方面的效果为100%。
Zotero 插件