van Iterson Vincent, Leidenius Marjut, von Smitten Karl, Bono Petri, Heikkilä Päivi
Breast Surgery Unit, Department of Oncology, Helsinki University Hospital, Helsinki, Finland.
Am J Clin Pathol. 2007 Nov;128(5):759-66. doi: 10.1309/7FXVRMXF58PVRJUH.
We assessed the expression of vascular endothelial growth factors (VEGF-C and VEGF-D) in breast cancer cells and the density of lymph vessels and VEGF receptor-3 (VEGFR-3)-positive vessels in and around the tumor in invasive lobular breast cancer. We found significant correlation between peritumoral lymph vessel density and presence of lymph node metastases (P=.001) and the number of metastatic lymph nodes (P<.001). A significant correlation was detected between tumor cell VEGF-D expression and lymph node status (P=.001) and density of lymphatic vessel endothelial receptor (LYVE)-1-positive vessels (P=.035). VEGFR-3+/VEGF-D+ and VEGFR-3+/VEGF-C+ tumors had a significantly higher number of metastatic lymph nodes than tumors with other staining patterns (P<.001). Tumors positive for neither VEGF-D nor VEGFR-3 had a lower density of LYVE-1+ vessels than tumors with other staining patterns (P=.033). Our results indicate that peritumoral lymph vessel density is associated with lymph node metastases in invasive lobular breast cancer and that invasive lobular cancer producing VEGF-D, surrounded by VEGFR-3+ vessels, has a significantly higher peritumoral lymph vessel density and a higher number of metastatic lymph nodes.
我们评估了浸润性小叶乳腺癌中癌细胞血管内皮生长因子（VEGF - C和VEGF - D）的表达，以及肿瘤内和周围淋巴管密度和VEGF受体-3（VEGFR - 3）阳性血管情况。我们发现肿瘤周围淋巴管密度与淋巴结转移的存在（P = 0.001）以及转移淋巴结数量（P < 0.001）之间存在显著相关性。在肿瘤细胞VEGF - D表达与淋巴结状态（P = 0.001）以及淋巴管内皮受体（LYVE）-1阳性血管密度（P = 0.035）之间检测到显著相关性。VEGFR - 3 + /VEGF - D +和VEGFR - 3 + /VEGF - C +肿瘤的转移淋巴结数量显著高于具有其他染色模式的肿瘤（P < 0.001）。VEGF - D和VEGFR - 3均为阴性的肿瘤，其LYVE - 1 +血管密度低于具有其他染色模式的肿瘤（P = 0.033）。我们的结果表明，肿瘤周围淋巴管密度与浸润性小叶乳腺癌的淋巴结转移相关，并且产生VEGF - D、被VEGFR - 3 +血管包围的浸润性小叶癌具有显著更高的肿瘤周围淋巴管密度和更多的转移淋巴结。
