Rosengren Sanna, Boyle David L, Firestein Gary S
Methods Mol Med. 2007;135:365-75. doi: 10.1007/978-1-59745-401-8_24.
The study of fibroblast-like synoviocytes (FLS) has yielded important insights into the pathogenic mechanisms of rheumatoid arthritis. FLS can be cultured from synovial tissue obtained at joint replacement surgery, synovectomy, or synovial biopsy. After collagenase digestion, adherent cells consist mainly of synovial fibroblasts and synovial macrophages. Proliferating FLS are enriched by repeated passage and comprise >95% of cells by passage 3. Because of cell senescence, use of FLS lines after passage 9 is generally not recommended. FLS in culture have a distinct phenotype with regard to morphology, ultrastructure, surface phenotype, and function. Surface markers that can be used to characterize FLS include positive staining for VCAM-1, CD44, CD55, CD90 (Thy-1), and cadherin-11, coupled with the absence of macrophage markers such as CD14 or CD68.
对成纤维样滑膜细胞（FLS）的研究为类风湿性关节炎的致病机制提供了重要见解。FLS可以从关节置换手术、滑膜切除术或滑膜活检获取的滑膜组织中培养得到。胶原酶消化后，贴壁细胞主要由滑膜成纤维细胞和滑膜巨噬细胞组成。通过反复传代可富集增殖的FLS，到第3代时其细胞占比超过95%。由于细胞衰老，一般不建议使用第9代以后的FLS系。培养中的FLS在形态、超微结构、表面表型和功能方面具有独特的表型。可用于鉴定FLS的表面标志物包括VCAM-1、CD44、CD55、CD90（Thy-1）和钙黏蛋白-11呈阳性染色，同时不存在如CD14或CD68等巨噬细胞标志物。
