Samper J C, Hellander J C, Crabo B G
Department of Animal Science, University of Minnesota, St. Paul 55108.
J Reprod Fertil Suppl. 1991;44:107-14.
Studies were designed to investigate whether sperm motility determined with a Hamilton-Thorn HTM-2000 motility analyzer (HTM), or the percentage of spermatozoa that passed through glass wool (GW), Sephadex (S), or glass wool/Sephadex (GWS) filters could be used to predict the fertilizing potential of fresh or frozen semen. In the fresh semen study, 10 randomly selected ejaculates from 4 stallions exclusively used for A.I. breeding were assayed during the season. The 521 mares used were inseminated with 500 x 10(6) motile spermatozoa after gynaecological examination every 2 days. In the frozen semen study, 32 ejaculates from 9 stallions were frozen and inseminated into 177 clinically and ultrasonographically normal mares within 12 h of ovulation with 10(9) spermatozoa, regardless of quality. In the fresh semen study, first cycle pregnancies ranged from 25-69% (average 50%) and differed among stallions (P less than 0.05), as did GWS, S, motile spermatozoa (MOT), mean progressive motility (MPV) and lateral head displacement (LHD) (P less than 0.001). GW and GWS filters, but not MOT, ranked the stallions according to fertility. The highest correlations were found between first cycle fertility and GWS (r = 0.86) and between that and MOT (r = 0.80). In the frozen semen study, first cycle and season pregnancy rates among the stallions were 32-70% (average 50%) and 60-90%, respectively. Significant correlations were obtained between the pregnancy rate per cycle and GWS (r = 0.93) and S (r = 0.84), but not between fertility and motility parameters.
研究旨在调查使用汉密尔顿 - 桑恩HTM - 2000精子活力分析仪（HTM）测定的精子活力，或通过玻璃棉（GW）、葡聚糖凝胶（S）或玻璃棉/葡聚糖凝胶（GWS）过滤器的精子百分比是否可用于预测新鲜或冷冻精液的受精潜力。在新鲜精液研究中，在繁殖季节对从4匹专门用于人工授精的种马中随机选取的10份射精进行了检测。所使用的521匹母马每2天进行一次妇科检查后，用500×10⁶个活动精子进行授精。在冷冻精液研究中，将9匹种马的32份射精进行冷冻，并在排卵后12小时内将10⁹个精子授精给177匹临床和超声检查正常的母马，而不考虑精液质量。在新鲜精液研究中，第一周期妊娠率在25% - 69%之间（平均50%），不同种马之间存在差异（P < 0.05），GWS、S、活动精子（MOT）、平均渐进性活力（MPV）和头部侧向位移（LHD）也存在差异（P < 0.001）。GW和GWS过滤器，但不是MOT，根据生育能力对种马进行了排名。第一周期生育能力与GWS之间的相关性最高（r = 0.86），与MOT之间的相关性次之（r = 0.80）。在冷冻精液研究中，种马的第一周期妊娠率和季节妊娠率分别为32% - 70%（平均50%）和60% - 90%。每周期妊娠率与GWS（r = 0.93）和S（r = 0.84）之间存在显著相关性，但生育能力与活力参数之间不存在显著相关性。
