棕色固氮菌合成海藻酸的过程。
The biosynthesis of alginic acid by Azotobacter vinelandii.
作者信息
Pindar D F, Bucke C
出版信息
Biochem J. 1975 Dec;152(3):617-22. doi: 10.1042/bj1520617.
Abstract
The sequence of reactions by which alginic acid is biosynthesized from sucrose in Azotobacter vinelandii was determined both by feeding radioactive individual enzymes involved. Results indicate that the first polymeric substance formed in the synthesis is polymannuronic acid and that mannuronic acid units are epimerized to guluronic acid at the polymer level. Guluronic acid does not appear to be formed at the monomer level, either free or in combination with GDP.
摘要
通过给参与其中的放射性单酶喂食，确定了棕色固氮菌中从蔗糖生物合成海藻酸的反应序列。结果表明，合成过程中形成的第一种聚合物质是聚甘露糖醛酸，并且甘露糖醛酸单元在聚合物水平上差向异构化为古洛糖醛酸。古洛糖醛酸似乎不是在单体水平上形成的，无论是游离形式还是与GDP结合的形式。
相似文献
1
The biosynthesis of alginic acid by Azotobacter vinelandii.棕色固氮菌合成海藻酸的过程。
Biochem J. 1975 Dec;152(3):617-22. doi: 10.1042/bj1520617.
2
Polysaccharide production and the possible occurrence of GDP-D-mannose dehydrogenase in Azotobacter vinelandii.棕色固氮菌中多糖的产生及GDP-D-甘露糖脱氢酶可能的存在情况。
Antonie Van Leeuwenhoek. 1975;41(1):25-32. doi: 10.1007/BF02565034.
3
Relationship between calcium and uroinic acids in the encystment of Azotobacter vinelandii.棕色固氮菌包囊形成过程中钙与尿酸的关系。
J Bacteriol. 1975 Apr;122(1):145-51. doi: 10.1128/jb.122.1.145-151.1975.
4
Identification and characterization of an Azotobacter vinelandii type I secretion system responsible for export of the AlgE-type mannuronan C-5-epimerases.负责输出AlgE型甘露糖醛酸C-5-表异构酶的维涅兰德固氮菌I型分泌系统的鉴定与特性分析。
J Bacteriol. 2006 Aug;188(15):5551-60. doi: 10.1128/JB.00236-06.
5
Hexuronyl C5-epimerases in alginate and glycosaminoglycan biosynthesis.藻酸盐和糖胺聚糖生物合成中的己糖醛酸C5-表异构酶。
Biochimie. 2001 Aug;83(8):819-30. doi: 10.1016/s0300-9084(01)01313-x.
6
Mannuronan C-5-epimerases and their application for in vitro and in vivo design of new alginates useful in biotechnology.甘露糖醛酸 C-5- 差向异构酶及其在体外和体内设计用于生物技术的新型藻酸盐中的应用。
Metab Eng. 1999 Jul;1(3):262-9. doi: 10.1006/mben.1999.0130.
7
Time-resolved 1H and 13C NMR spectroscopy for detailed analyses of the Azotobacter vinelandii mannuronan C-5 epimerase reaction.用于详细分析棕色固氮菌甘露糖醛酸C-5差向异构酶反应的时间分辨1H和13C核磁共振光谱法。
Biochim Biophys Acta. 2002 Mar 15;1570(2):104-12. doi: 10.1016/s0304-4165(02)00195-2.
8
Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.威氏固氮菌中产生的褐藻胶中长古洛糖醛酸嵌段的生物合成与功能。
Biomacromolecules. 2019 Apr 8;20(4):1613-1622. doi: 10.1021/acs.biomac.8b01796. Epub 2019 Mar 19.
9
Guluronic acid content as a factor affecting turbidity removal potential of alginate.糖醛酸含量作为影响褐藻酸钠浊度去除潜力的因素。
Environ Sci Pollut Res Int. 2016 Nov;23(22):22568-22576. doi: 10.1007/s11356-016-7475-6. Epub 2016 Aug 24.
10
Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family.三种与固氮菌 AlgE 甘露聚糖 C-5-差向异构酶家族相关的节杆菌褐藻胶修饰酶的功能特征。
Sci Rep. 2020 Jul 27;10(1):12470. doi: 10.1038/s41598-020-68789-3.
引用本文的文献
1
A mutant of phosphomannomutase1 retains full enzymatic activity, but is not activated by IMP: Possible implications for the disease PMM2-CDG.磷酸甘露糖变位酶1的一种突变体保留了完整的酶活性，但不受肌苷酸（IMP）激活：对疾病磷酸甘露糖变位酶2缺陷型先天性糖基化障碍（PMM2-CDG）的潜在影响
PLoS One. 2017 Dec 19;12(12):e0189629. doi: 10.1371/journal.pone.0189629. eCollection 2017.
2
Biological function of a polysaccharide degrading enzyme in the periplasm.多糖降解酶在周质空间中的生物学功能。
Sci Rep. 2016 Nov 8;6:31249. doi: 10.1038/srep31249.
3
Comparative characterization of two GDP-mannose dehydrogenase genes from Saccharina japonica (Laminariales, Phaeophyceae).来自海带（海带目，褐藻纲）的两个GDP-甘露糖脱氢酶基因的比较特征分析
BMC Plant Biol. 2016 Mar 8;16:62. doi: 10.1186/s12870-016-0750-3.
4
Production of polyhydroxybutyrate and alginate from glycerol by Azotobacter vinelandii under nitrogen-free conditions.棕色固氮菌在无氮条件下由甘油生产聚羟基丁酸酯和藻酸盐。
Bioengineered. 2015;6(4):209-17. doi: 10.1080/21655979.2015.1040209. Epub 2015 Apr 16.
5
Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii.从土壤杆菌中鉴定出 AlgE4 和 AlgE6 两种海藻酸 C-5 差向异构酶的结构与功能特征。
J Biol Chem. 2014 Nov 7;289(45):31382-96. doi: 10.1074/jbc.M114.567008. Epub 2014 Sep 29.
6
The Pseudomonas aeruginosa AlgZR two-component system coordinates multiple phenotypes.铜绿假单胞菌AlgZR双组分系统协调多种表型。
Front Cell Infect Microbiol. 2014 Jun 20;4:82. doi: 10.3389/fcimb.2014.00082. eCollection 2014.
7
Bacterial exopolysaccharides: functionality and prospects.细菌胞外多糖：功能与前景
Int J Mol Sci. 2012 Oct 30;13(11):14002-15. doi: 10.3390/ijms131114002.
8
Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.革兰氏阴性菌中依赖合酶的胞外多糖分泌。
Trends Microbiol. 2013 Feb;21(2):63-72. doi: 10.1016/j.tim.2012.10.001. Epub 2012 Oct 29.
9
Lipoglycans contribute to innate immune detection of mycobacteria.糖脂类参与分枝杆菌的固有免疫检测。
PLoS One. 2011;6(12):e28476. doi: 10.1371/journal.pone.0028476. Epub 2011 Dec 2.
10
Alginate production and alg8 gene expression by Azotobacter vinelandii in continuous cultures.在连续培养中，固氮菌属（Azotobacter vinelandii）产生海藻酸盐和 alg8 基因的表达。
J Ind Microbiol Biotechnol. 2012 Apr;39(4):613-21. doi: 10.1007/s10295-011-1055-z. Epub 2011 Nov 10.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
3
EXTRACELLULAR POLYSACCHARIDES OF AZOTOBACTER VINELANDII.棕色固氮菌的胞外多糖
J Bacteriol. 1964 Aug;88(2):329-38. doi: 10.1128/jb.88.2.329-338.1964.
4
The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
J Biol Chem. 1959 Aug;234(8):1971-5.
5
Direct demonstration of ammonia as an intermediate in nitrogen fixation by Azotobacter.直接证明氨是固氮菌固氮过程中的一种中间产物。
J Biol Chem. 1953 Sep;204(1):445-51.
6
Pathway of algnic acid synthesis in the marine brown alga, Fucus gardneri Silva.海洋褐藻加氏墨角藻中褐藻酸的合成途径。
J Biol Chem. 1966 Nov 25;241(22):5284-97.
7
Determination by a kinetic method of the nearest-neighbour frequencies in a fragment of alginic acid.用动力学方法测定海藻酸片段中相邻频率。
Acta Chem Scand. 1969;23(7):2375-88. doi: 10.3891/acta.chem.scand.23-2375.
8
Biosynthesis of alginate. II. Polymannuronic acid C-5-epimerase from Azotobacter vinelandii (Lipman).藻酸盐的生物合成。II. 来自棕色固氮菌（利普曼）的聚甘露糖醛酸C-5-差向异构酶
Carbohydr Res. 1971 Apr;17(2):297-308. doi: 10.1016/s0008-6215(00)82537-9.
9
Biosynthesis of alginate. 1. Composition and structure of alginate produced by Azotobacter vinelandii (Lipman).藻酸盐的生物合成。1. 棕色固氮菌（利普曼）产生的藻酸盐的组成与结构。
Carbohydr Res. 1971 Apr;17(2):287-96. doi: 10.1016/s0008-6215(00)82536-7.
10
Polyacrylamide gel electrophoresis of alginic acid.海藻酸的聚丙烯酰胺凝胶电泳
J Chromatogr. 1974 Feb 13;89(1):99-102. doi: 10.1016/s0021-9673(01)84167-4.
Zotero 插件