经口给予胸腺五肽负载的N-三甲基壳聚糖纳米粒的研究
[Research on thymopentin-loaded N-trimethyl chitosan nanoparticles administered through mouth].
作者信息
Tang Shi-wei, Yuan Xiao-jia, Zhang Zhi-rong, Song Qing-guo, He Qin
机构信息
Key Laboratory of Drug Targeting, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Sep;38(5):885-8.
OBJECTIVE
To prepare a peroral thymopentin-loaded N-trimethyl chitosan chloride-nanoparticle (Tp5-TMC-NP) ,and observe the pharmacodynamic action when the Tp5-TMC-NP is taken by way of the mouth.
METHODS
N-trimethyl chitosan chloride was first synthesized, and then Tp5-TMC-NP was prepared with the formulation technology optimized by the Central Composite Design. The influence of Tp5-TMC-NP on the ratio of CD4+/CD8+ of T-lymphocytes were determined by flow cytometer.
RESULTS
The regular global Tp5-TMC-NP prepared with the optimized formulation craft had the mean diameter of 110.6 nm and got the entrapment efficiency of 78.8%. The ratio of lymphocyte CD4+/CD8+ of Wistar rat administered with Tp5-TMC-NP perfusing stomach had 2.59 times higher than that with Tp5.
CONCLUSION
Taken orally the Tp5-TMC-NP has much higher efficiency than Tp5.
目的
制备口服胸腺五肽负载的氯化N-三甲基壳聚糖纳米粒（Tp5-TMC-NP），并观察Tp5-TMC-NP经口给药后的药效学作用。
方法
首先合成氯化N-三甲基壳聚糖，然后采用中心复合设计优化的制剂技术制备Tp5-TMC-NP。用流式细胞仪测定Tp5-TMC-NP对T淋巴细胞CD4+/CD8+比值的影响。
结果
采用优化后的制剂工艺制备的规整球形Tp5-TMC-NP平均粒径为110.6nm，包封率为78.8%。灌胃给予Tp5-TMC-NP的Wistar大鼠淋巴细胞CD4+/CD8+比值比给予Tp5的大鼠高2.59倍。
结论
口服Tp5-TMC-NP的效率比Tp5高得多。
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